The Reserach On Relationship Between Eggshell Color, Composition, Structure And Eggshell Quality

In order to explore the relationship between eggshell quality and eggshell color, composition and structure, choose 98 eggs laid by New Yangzhou chickens, eggshell quality traits was first determinated. Then eggshell color was determinated by QCR eggshell color instruments to explore the deposition of eggshell pigment. Then collect the eggshell in test one to determinate shell composition, final select five eggshells in accordance with intensity to observate microstructure and crystal structure. The results showed that: 83.673 percent of New Yangzhou chicken eggs were light brown. Eggshell weight of brown eggs was 5.87±0.22 g, was the highest, the proportion of eggshell weight in egg weight of brown eggs was 12.77±1.34, was the highest, egg weight of brown eggs was 49.20±4.59 g, was also the highest, shell thickness of brown eggs was 0.340±0.027 mm, was also thicker than other groups; Eggshell rate was 12.30±1.15%; Eggshell thickness was 0.306±0.033 mm; Egg shape index was 0.762±0.039; Harrington Unit was 81.35±10.88. The eggshell strength had a very high significant positive correlation (P<0.01) with eggshell thickness, shell weight, shell ratio, shell weight per unit area, the eggshell thickness and strength had a bigest correlation coefficient 0.717. Egg weight and eggshell strength had significantly negative correlation (P<0.01). Pigment deposition in eggshell: biliverdinⅨand protoporphyrinⅨwere reached the largest secretion efficiency in final stage of eggshell forming, they may be subject to the same control factors. There were certain relationships between eggshell color and egg weight, eggshell strength, eggshell thickness and Harrington units. Egg weight of shell color 45~50 had significant difference with other groups(P<0.05). Eggshell strength of color 30~35 was significantly higher than other groups(P<0.05). Eggshell thickness of color 30~35 and 35~40 and eggshell thickness of color 40~45 and 45~50 had significant difference(P<0.05). Eggshell Harrington units of color 45~50 had significant difference with the first three groups (P<0.05). The determination of eggshell components results showed that: There was not significant difference between calcium content of different strength in eggshell(P>0.05). Phosphorus, carbon, oxygen, nitrogen relative content of different intensity were not significant (P>0.05). Protein determination results showed: Also the difference was not significant (P>0.05),but eggshell strength and thickness were first increased and then declined increased with the protein content. Concentrations of uronic acid and sulfated GAGs were negatively correlated with eggshell strength in calcified shell (P<0.01), concentrations of uronic acid was weak positively correlated with eggshell strength in shell membrane,concentrations of sulfated GAGs was positively correlated with eggshell strength in shell membrane(P<0.01). Ultrastructural observation showed that: The effective thickness of mastoid layer and mastoid gap of high intensity treatment were lower than middle intensity and low intensity treatments by 32.43%(P<0.01) and 26.93%(P<0.05), 71.71%(P<0.01) and 36.08%(P<0.05), respectively. Obtain New Yangzhou chicken eggshell diffraction patterns by diffraction experiment. Spacing and diffraction intensity of different eggshell strength groups were similar.