Research Of The Requirement Of Copper For Male Sika Deer In Body Growing And Antler Growing Periods

Used feeding trials and digestion test, and combined with biochemical analysis and evaluation of serum biochemical parameters and production performance, this paper was to investigate the most appropriate form of copper added into feed for growing male sika deer. Through evaluating the effects different levels of copper on the digestibility of nutrients, production performance and serum biochemical parameters for sika deer to ascertain the optimum levels of copper added into feed.Experimentâ… : In order to investigate the effects of different copper sources on serum biochemical parameters and the nutrients digestibility in growing male sika deer, Corn flour, soybean meal, wheat bran, alfalfa, corn stalk, DDGS, salt and additives were premixed as the basic diet. Twenty growing male sika deer were allotted randomly to four groups (A, B, C, D) at the same level of nutrients and similar concentration of energy. Group A was fed with basic diet without additional copper. Group B, C and D were experimental groups and fed with basic diets added 10 mg/kg copper sulfate, copper citrate and methionine copper respectively. The aim of the study was to investigate the effects of different forms of copper on serum biochemical parameters and the digestibility of nutrients in growing male sika deer and then ascertained the optimum copper source during growing period.Experiment II: For researching the effects of methionine copper on growing male sika deer, Corn flour, soybean meal, wheat bran, alfalfa, corn stalk, DDGS, salt and additives were premixed as basic experimental diet. Twenty growing male sika deer allotted randomly to four groups (A, B, C, D) at the same level of nutrients and similar concentration of energy. Group A was fed with basic diet without additional copper. Group B, C and D were experimental groups and fed with diets added 15, 40 and 80 mg/kg methionine copper respectively. The aim of the design was to investigate the effects of different levles of methionine copper on serum biochemical parameters and the digestibility of nutrients in growing male sika deer and then ascertained the optimum levels of copper added into feed during growing period.Experiment III: For researching the effects of methionine copper on male sika deer in antler- growing period, Corn flour, soybean meal, wheat bran, alfalfa, corn stalk, DDGS, salt and additives were premixed as the basic experimental diet. Twenty growing male sika deer were allotted randomly to four groups (A, B, C, D) at the same level of nutrients and similar concentration of energy. Group A was fed with basic diet without additional copper. Group B, C and D were experimental groups and fed with diets added 15, 40 and 80 mg/kg methionine copper respectively. The aim of the design was to investigate the effects of different levles of methionine copper on serum biochemical parameters and the digestibility of nutrients and production performance and then ascertained the optimum range of copper added into feed during antler-growing period.The results were as follow:1. Copper added into diet could improve the activities of enzyme that relate to copper and the digestibility of some nutrients. The optimum copper source during growing period for sika deer was methionine copper.2. Copper added into diet during growing period could improve the digestive function in the gastrointestinal tract of sika deer. The digestibility of neutral detergent fiber had significant change compared with the control group when 40mg/kg cooper added into diet (P<0.05); the digestibility of crude protein and phosphorus had significant difference when 15mg/kg copper added into diet (P<0.01); Copper added into diet could inrease copper content in serum significantly during growing period (P<0.05); The activity of ceruloplasmin had significant change when 40 mg/kg copper added into diet in growing period (P<0.05). The appropriate copper content added into diet in growing period in Jilin area was from 15 to 40 mg/kg. Totle amount of copper in diet was from 21.21 to 45.65 mg/kg.3. Copper added into diet during growing period could improve the digestive function in the gastrointestinal tract of sika deer; The activity of ceruloplasmin had significant change when 15 mg/kg and 40 mg/kg copper added into diet during antler growing period (P<0.05); Antler production increased with the gradual increase of copper. The production reached the maximum when 40 mg/kg copper added into diet; Copper added into diet could promote the content of amino acids in antler. The appropriate copper content added into diet during sika deer antler growing period in Jilin area was 40 mg/kg .Totle amount of copper in diet was about 46.09 mg/kg.