Two Kinds Of Botanical Extracts On The Effect Of Insect-control Potential Of The Wolf Spider Pardosa Pseudoannulata

By using ultraviolet spectroscopy, acetylcholinesterase (AChE) activity in the wolf spider Pardosa pesudoannulata body in different dose physiological active materials to the acetylcholinesterase-catalyzed hydrolyzation of acetylcholine and their activity effect were systemically researched. The results showed that the hydrolyzation was significantly inhibited in the presence of physiological active materials. The present work may have presented a novel method feasible for the study on the effect of low-dose physiological active materials on the neural excitability of spiders.Digestive enzyme activities of the wolf spider, Pardosa pseudoannulata were determined by means of electronmicroscopy and biochemical analysis. The results of biochemical analysis showed the activities of protease and lipase of the wolf spider had significant changes under the fittest low-dose physiological active materials compared with those of the normal. Under the fittest low-dose physiological active materials, the activities of protease and lipase of the wolf spider is significantly increased.The functional reponse and searching behavior to the brown planthopper(BPH), Nilaparvata lugens by its predator, the wolf spider Pardosa pseudoannulata treated with the physiological active materials of 3 different concentrations in plants were studied in the laboratory. The results showed that the predating number of the predator to the prey was significantly higher under the fittest concentration physiological active materials (5:50) than those without treatment or treated by high-dose concentration. Although all predating functional responses were in accordance with the Hollingâ…¡model, the functional response of the spiders treated by the fittest physiological active materials in plants was obviously stronger than those without treatment or treated by high-dose physiological active materials. The results dedicated preliminary that these physiological active materials could be used as an attraction agent of spiders.