| The exploitation of Chinese herb immunopotentiators is a hot spot of modern pharmacy of Chinese herb medicine and modern immunology. This research's aim is to develop an effective Chinese herb immunopotentiator based on a series of vivo and. vitro tests.(1) selection of the complex prescription and the effect on the non-specific immunofunction in miceVivo tests in mice were done to select the most effective prescription of Chinese medicine immunopotentiators from 6 given complex Chinese herb medicine prescription. The results showed that:prescription A could extend the mice's weight-carrying swimming time significantly,20-27.5% more than the control groups; prescription B could extend the mice's weight-carrying swimming time, increase the number of mononuclear cells and the CH50 significantly, and the increasing amounts were respectively 28.4-39.5%,2.8-94.4% and 0.8%-39.9% than the control groups; prescription C could extend the mice's weight-carrying swimming time, increase the number of white blood cells and neutrophilic granulocytes, the phagocytosis activity of mononuclear macrophage, the LZM enzyme's activity and the content of IFN-αin serum significantly, and respectively increased 6.4-25.0%,2.4-89.6%,12.2-27.9%,4.1-23.4%,45.8-65.0%,18.0-40.6% than control groups; prescription D could extend the mice's weight-carrying swimming time, increase the number of white blood cells, lymphocytes, mononuclear cells and neutrophilic granulocytes, improve the phagocytosis activity of mononuclear macrophages and the LZM enzyme's activity significantly, and the increasing amounts were respectively 22.6-39.5%,15-94.8%,11.1-111.1%,56-88%,8.7-107.5%,5.7-34.3% and 9.84-233.9% compared with the control groups; prescription E could extend the mice's weight-carrying swimming time, increase the number of white blood cells, lymphocytes, mononuclear cells, and neutrophilic granulocytes, improve the phagocytosis activity of mononuclear macrophages and the LZM enzyme's activity significantly, and the increasing amounts were respectively 15.8-25.6%,5.2-110%,2.7-88.4%,10-132%,18-114.1%,6.2-26.9%, and 108.4-152.3% compared with control groups; while, prescription F could extend the mice's weight-carrying swimming time, increase the index of immune organ, improve the LZM enzyme's activity and the CH50 significanlyt, and the increasing amounts were respectively 9.6-35.6%,31.3-108.4%,39.7-166.7% and 7.1-8.4% compared with the control groups. Thus prescription C was selected as the best prescription of the "Zengkangling" after comprehensive analysis.(2) Effect on specific immunofunctionIn order to study the effect of "Zengkangling" on the immunofunction of Newcastle disease(ND) vaccine and hog cholera(HC) vaccine, the experimental animals were divided into four groups:high, medium, low dose and control groups, and detected the valence of corresponding antibody in serum. The results showed that: the chickens of the high dose group reached the peak of valence of antibody at 12th day after inoculated with ND vaccine for the first time, and the continued period of high level of antibody valence above 100 could reach 7 days; and the time when the valence of antibody reach the peak was the 10th day after inoculated the second time, and the corresponding continued period could last for 21 days. Meanwhile, The piglets of all trial groups inoculated the HC vaccine for the first time, reached the peak of valence of antidody after 2 weeks, the high dose group's continued period of high OD(level of valence, OD≥0.4) was the longest one lasting for 5 weeks. The results indicated that "Zengkangling" could enhance the animal's specific immunofunction significantly.(3) Antibacterial tests in vitroThe MIC of "Zengkangling" to Escherichia coli, Salmonella, Pasteurella and Staphylococcus aureus were respectively 875μg·mL-1,218.75μg·mL-1,437.5μg·mL-1 and 875μg·mL-1 and the MBC to those were respectively 875μg·mL-1,437.5μg·mL-1, 875μg·mL-1 and 875μg·mL-1 through test tube method. The results indicated that "Zengkangling" have certain antibacterial activity in vitro.(4) Anti-virus test in vitro.To evaluated the anti-PR V effect of "Zengkangling" in vitro,3 ways were adopted:one way was PRV added alone onto the PK-15 cells before"Zengkangling", the second way was "Zengkangling" added before PRV, and the third way was PRV and "Zengkangling" added together after mixed and Interacting for 2 hours. With Observing the cytopathic effect of PK-15 cells originated by PRV, and calculating the results with Reed-Muench Method, the TDo was 138.6μg/mL; TD50 was 353.6μg/mL, IC50 were 2.8,5.5 and 12.8μg/mL, and TI were 126,64 and 28. The results indicated that "Zengkangling" have certain anti-virus activity in vitro.The results of above-mentioned showed that Chinese herb complex immunopotentiators "Zengkangling" could enhance the immunofuction of animals, and possessed certain antibacterial activity together with anti-virus activity in vitro. Thus it was an immunopotentiator with multi-pharmacological activities.
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