Genetic Basis Of Grain Appearance Quality By Using Chuanxiang 29B/Lemont Recombinant Inbred Line

Rice appearance quality had been becoming one of the most important traits in the rice breeding program.It was mainly referred to grain length (GL),grain width(GW),grain thickness(GT),ratio of length and width( L/W ) and chalkiness(CH). In the paper, a recombinant inbred line population with 184 lines was developed, which was derived from a cross between Chuanxiang 29B (Oryza sativa indica) and Lemont (Oryza sativa japonica),The quantitative trait loci(QTL) for six appearance traits including grain length(GL), grain width(GW) , grain thickness(GT),grain weight (GW, chalkiness(CH). and grain length-width ratio (GLW) were mapped in two environments (Chengduand Hainan).The main results were as follows:(1)A genetic linkage map, contained 98 SSR markers, was constructed. The map covered a total of 1244.8cM with an average interval of 17.25cM.(2) The correlation analysis among rice appearance traits indicated that,RGT had a highly significant positive correlation with RGL,RGW;KGW had a highjly significant positive correlation with RGL,RGW and RGT;PCG had a highly significant positive correlation with RGW,KGW,However,RCG had a significant negative correlation with RGL,RCG had a significant positive correlation with RGT;L/W had a highly significant negative correlation with RGW,PCG,L/W had a significant negative correlation with RGT,KGW;However, L/W had a highly significant positive correlation with RGL in the same enviroment(3)The total of 18 QTLs distributed on eleven chromosomes were detected, including 9 QTLs in Hainan experimental point and 9 QTLs in Chengdu experimental point,and 3 QTLs in both Hainan and Chengdu experimental point ,locating on chromosome 2,3,4,5,6 and 7.(4) There was the QTLs cluster distribution on chromosomes , On the 7rd chromosome, a QTLs cluster for RGW and KGW was located between RM478 and RM351. A QTLs cluster for RGW and KGW on the 5th chromosome (RM164-RM3437) was identified. There was also a QTLs cluster for RGW,RGL and L/W on the 7th chromosome (RM351-RM336).