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Selection And Breeding Of Hyperthermal Deodorizating Complex Microorganism And Its Application On Pig Manure Composting

Posted on:2011-12-21Degree:MasterType:Thesis
Country:ChinaCandidate:X W CengFull Text:PDF
GTID:2143360308972180Subject:Plant Nutrition
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Ammonia was the main odor during pig manure composting. The volatilizarion of ammonia not only polluted environment but also caused significant loss of nitrogen, resulting in composting quality decrease. One of the main factors constrained the production of composting was that the time of decomposition spent was too long. The basic way of deodorization is based on microoganism. How to control the losses nitrogen and screening the high-efficiency strains is becoming an ever-increasing important issue.To study the aerobic thermostabilization and deodorization, microorganism were separated from horse manure, rabbit manure, straw-cattle manure, mushroom residue and pig manure mature composting. Selection of the strains which could promote composting and reduce the volatilization of NH3 was studied,either.The results were showed below:1. Ninety hyperthermal strains were isolated from the five materials, including forty-seven bacteria, twenty-two actinomycetes and twenty-one fungus. The counting results showed that bacteria was the predominant microbe in each material. The quantity of hyperthermal microbes in horse manure was the most and followed by rabbit manure.Twenty-six no-ammonia and less-ammonia strains were selecting out by a screening test of producing ammonia, including thirteen bacteria (accession numbers:X1~X13), seven actinomycetes(accession numbers:F1~F7) and six fungus (accession numbers:Z1~Z6).2. The twenty-six deodorized strains had great difference effect during pig manure composting. All strains reduced the volatilization of NH3 effectively except X6. The inhibition ratio of NH3 volatilization of these treatments such as "X8" and "F2" and "Z2" and "Z3" and "Z4" and "Z6", was higher more than 70%, compared with control. The best one was the "X8" treatment, which inhibition ratio of NH3 was 84%. All strains could accelerate pig manure decomposing except "X1", "X2", "X4", "X6", "X9", "X10", "X13", "F4", "F6", "Z1" and "Z2". Considering all indexs, the best strain was "X8", followed by "F2" and "Z6" strains.There was no antagonism among the three strains,then mixed them to construct a complex microorganism named 1#.3. Comparing the effect among "X8", "F2", "Z6" and 1# inoculated during the process of pig manure composting, we found that the best one was 1#, its inhibition ratio of NH3 volatilizing was 74%, "X8" was 70%, "F2" was 65.5%, and "Z6" was 62.5%. At the end of composting, the accumulation of total nitrogen (TN) in these microbial agent treatments was increased. The rate of increase was 9.8% in the 1# agent, and was the highest. "X8", "F2", and "Z6" treatment were 7.6%,6.8% and 5.2% respectively. The treatments added microbial agent were significantly different comparing with the control, but no significant difference between "X8" and "F2" treatment. The seed germination index (GI) was determined, the results showed that all four treatments of inoculating microbes could speed up composting. The 1# treatment showed that the compost reached the maturity state on 15th day, compared with the control's maturity time of 7 days in advance.The complex 1# could be used to pig manure composting with its ability of high-effective deodorant and greatly shorten the time of compost maturity.
Keywords/Search Tags:Pig manure, Composting, Hyperthermal microorganism, Deodorization
PDF Full Text Request
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