The Changes Of Muscle Fibers And MyoG MRNA Expression In Muscles Of ND-immunized Chicks With Probiotics

Probiotics, in the animal digestive tract, has a dominate role of beneficial bacteria, they can take nutrients competition with harmful bacteria to maintain the balance of microbial flora for mature of chick immune organs, and enhance immune function, improve stress resistance, broiler performance. The experiment was conducted by using of one-day-old chicks. The growth performance, meat quality indicators, muscle fiber structure and MyoG mRNA expression of probiotics-fed chicks, ND-vaccinated and probiotics-fed chicks and following challenged with NDV were examined by molecular biology technology and organization, ultrastructure detection. The results were as follows:Chicks were fed with probiotics an one day age and changes of growth performance and meat quality were studied. The body weight of chicks post fed was significantly higher than in control unfed group chicks, and forage/flesh proportion was lower than them at 7 days later (p<0.05, p<0.01). Meat indicators are much higher than the control chicks unfed, showed that probiotics improve chick performance as soon as meat quality. The muscle density and lipid droplets in muscle fiber of chicks with probiotics was significantly higher than that of control chicks, and muscle fiber diameter decreased compared with control chicks, showed that chicks fed with probiotics regulate the growth of muscle fibers and adipose tissue formation was significantly enhanced. The expression of MyoG mRNA in muscle increased from 7 to 14 day post fed, then gradually decreased, while the slower rate of decline in probiotics-fed chicks were the control chicks, the expression of MyoG mRNA could be with nutritional status of the chicks.ND-vaccinated chicks with probiotics, its growth performance, meat quality and the growth of muscle fibers are better than those without probiotics (p<0.05, p<0.01), ND-vaccinate chicks was no significant difference with control chicks. Account for the above-mentioned examples, it could be show that ND vaccine on chicks growth performance and muscle was no obvious effect on muscle fiber development. The expression of MyoG mRNA of chick with probiotics decrease more slowly without unfed probiotics may be relate to nutrition metabolism.All of the probiotics-fed and ND-vaccinated chicks were successfully protected post challenged with NDV. At the meantime, ND-vaccinated chick with probiotics on growth performance, meat quality is better than alone ND-vaccinated and probiotics-fed chicks. Muscle growth, muscle fibers and MyooG mRNA expression of probiotics-fed chicks increased significantly than control chicks (p<0.01). In addition to a secondary that probiotics enhance the activation of ND vaccine organism acquired immune function, can also enhance the stability of metabolism after virulent attack, chicks of the ND vaccine can enhance the immune response and reduce the incidence of chick, enhanced resistance to virus attack.All of the results suggested that the first time methods of muscle fiber structure detection and molecular technique revealed the probiotics could improve overall production performance and optimize meat quality of chick, a scientific theory is provided for use and development of probiotics.