Studies On Utility Position And Antibacterial Drug Efficacy Of Different Ecucalytypus Species

To explore antimicrobial activity of effective position on different Eucalytyptus species'leaves which extracted by organic solvent, security of main species'effect on growth performance,immune and anti-oxidation of broiler chicken, and provide research-based for promoting and using in animal production. The flat plate punch method and test tube double dilution method were used to determine the bacteriostatic effects in vitro of DH201-2,GL-12,GL-16,DH32-29,GL-9 and U6 six kind of Ecucalytypus leaves which of different treatment and which were had bacteroistatic effect on B. cereus, S. aureus, P.multocida and V. Parahaemolyticus. Using two-phase solvent extraction to isolated different extracted components from GL-9 and U6 Ecucalytypus species, and observe bacteriostatic effect on E.coli, B. cereus, S. aureus, S. enteritidis, P.multocida, V. Parahaemolyticus, V.alginolyticus and V.cholera by different extracted components. Radial filter paper chromatography method was used to detect the component of each composition, then using different extract methods to get the antibacterial activity materials and tested their drug susceptibility in vitro; using orthogonal trains to explore optimum conditions and influencing factors of effective antimicrobial part; according to the requirement of acute toxicity experiment to tested LD50 lethal dose of GL-9 and U6 Ecucalytypus leaves.150 1-day-old broiler Chicken were divided into five groups randomly: control groupⅤ, tested groupsⅠ,Ⅱ,Ⅲwhich were respectively supplied with U6 Ecucalytypus leaves power additive feedstuff at 0.25%, 0.5% ,1%, and groupⅣwas 0.5%Shuanghuanglian. The chicken weight and feed intake was measured every week, and calculated growth performance of ADG,ADFI and F/I , and measured the indexes of ACP, AKP,SOD,GHS-pX and MDA. Results showed:1. Decoction , decoction extract, alcohol extract, ethanol extract of different varieties of Ecucalytypus leaves had an different inhibition effect on four kinds of tested strains, the inhibition effective against pasteurella was the strongest and the diameter of bacteriostasis circle of pasteurella which was 22.73 ~ 36.79mm much larger than Shuanghuanglian group, 13.21mm.2. The diameters of bacteriostasis circle of Decoction, decoction extract, alcohol extract and ethanol extract were respectively 11.34～36.79mm,6.36～33.97mm,9.69～33.56mm,6.60～32.66mm. 3. The diameters of bacteriostasis circle of GL-9 and U6 Ecucalytypus leaves'decoction were better than other four species and respectively 15.35～34.40 mm,14.71～32.24 mm.4. The lower component of GL-9 and U6 Ecucalytypus leaves'decoction extraction had a strongly sensitive effect on the 8 tested strains which were E.coli, B. cereus, S. aureus, S. enteritidis, P.multocida, V. Parahaemolyticus , V.alginolyticus and V.cholera. And the inhibited effect equaled with the decoction liquid, antibacterial effect of ethylacetate and n-butanol components relatively weak, chloroform and petroleum ether components were not showed any effect, so that the antibacterial effective part of GL-9 and U6 Ecucalytypus leaves mainly in the lower component of the extraction.5. To detected the component of decoction, ethyl acetate layer, n-butanol layer about GL-9 and U6 Eucalytyptus leaves showed that they contained multiple active ingredients, respectively as phenols, Steroid, flavonoids, terpenoids, saponins, amino acid compounds. And these active ingredients could not be effectively separated and the inhibition effect was less than the water extraction of GL-9 and U6 Ecucalytypus leaves. Antimicrobial spectrum of phenols and flavonoids consistented with the performance of water-extract.6. The experiment results which was different extraction condition of GL-9 and U6 Ecucalytypus leaves showed extracting time and temperature were mainly factors of influencing antibacterial effect on GL-9 Ecucalytypus leaves, and extracting time had a greater impact on antibacterial effect of U6 Ecucalytypus leaves.7. LD50 of GL-9 Ecucalytyptus leaves was 47.3696 g/kg, LD50 of U6 Ecucalytypus leaves was 68.7702 g/kg.8. Added U6 Ecucalytypus leaves to broiler'diet have no effect on growth performance and nonspecific immunity; diet supplemented with 0.5% U6 Ecucalytypus leaves significantly increased glutathione peroxidase (GSH-PX) activity, to maintain MDA content in the lower level, and on superoxide dismutase (SOD) had no effect.In conclusion, different species Ecucalytypus leaves had vitro bacteriostatic action on common pathogenic bacteria,different extract modus had no significantly effect on bacteriostatic action and water extraction liquor'vitro bacteriostatic action was high to alcohol extraction liquor; the vitro bacteriostatic action of GL-9 and U6 Ecucalytypus was better than the other four species. The underlayer component was the main position of bacteriostatic action on GL-9 and U6 Ecucalytypus, flavonoids and phenolic compounds were the main vitro bacteriostatic active material, the significiant effect facter of extracting position of bacteriostatic action were extracting temperature and time, for U6 was extracting time. GL-9 Ecucalytypus was mild toxicity, U6 Ecucalytypus was asepsis. Added U6 Ecucalytypus leaves to broiler'diet have no effect on growth performance and nonspecific immunity, but could improve the anti-oxidation ability, addition of 0.5% was suitable.