Effect Of Different Agronomic Measures On Basal Aroma Components And Related Character In Flue-cured Tobacco Leaf

Our research aimed to explore the reasons of the impact of tobacco flavor material produced for the differences among varieties and the best control measures ,we have studied the new variety of the flue-cured 8306 and H892, and conventional cultivars K326 and ZY 100. the impact was resear -ched on the content of aroma and composition by two control measures of nitrogen levels and the outer of the salicylaldehyde acid treatment, the preliminary conclusions are as follows:1 Chlorophyll and carotenoid content in the tobacco leaf increased with growing flue-cured tobacco leaf, it reached the highest value at 20d or 30d , then gradually decreased, all genotypes showed the same tendency. chlorophyll content and carotenoid content were significantly different among the genotypes. On the same leaf age Chlorophyll content of H892 and 8306 were significantly higher than K326 and ZY100 ,carotenoid content of H892 was significantly higher than the other three genotypes, carotenoid content of 100 lower than the other three genotypes. Among nitrogen levels content of chlorophyll and carotenoid content were significantly different. Increase of the amount of nitrogen will help improve the chlorophyll content, the chlorophyll content of the four genotypes in various ages showed: high nitrogen treatment > ordinary nitrogen treatment > low nitrogen treatment, differences were significant. Carotenoid content of nitrogen treatments was significantly different, ordinary nitrogen treatments > the highest nitrogen treatment > low nitrogen treatment, the appropriate amount of nitrogen can improve the content of carotenoids.The difference of the chlorophyll and carotenoid content among nitrogen treatments and genotypes showed that the control to the right of chlorophyll and carotenoid content is feasible by change of genotypes or nitrogen regulation.The results show that:On the tobacco leaf the best concentration of salicylic acid treatment was 1.5mmol·L^-1. The change of spraying salicylic acid treatment and CK of chlorophyll content and carotenoid content was a single peak curve, but different variation between spraying salicylic acid treatment and CK . At the 3d-6d chlorophyll and carotenoid content of spraying SA treatment has been higher than CK, after spraying 6d chlorophyll and carotenoid content reached the top.At 15d, the chlorophyll content was 1.13 times than CK, carotenoid content was 1.10 times than CK.2 Glandular hairs density showed a downward trend with growing flue-cured tobacco leaf, it reached the highest at 20d, and lowest at 60d. A significant difference among genotypes, glandular hairs of 8306 are denser than K326's. Nitrogen treatment on the glandular density was significantly difference, glandular hairs showed the density of ordinary nitrogen treatment> high nitrogen> low nitrogen treatment.3 The change of overall genotypes tobacco leaf surface trichome exudate showed single peak with growing flue-cured tobacco leaf, increased in 20d-40d, peaked at 40d and then decreased, decreased slowly in 50d-60d. At 40d content were significant differences among genotypes, 8306> H892> ZY100> K326. At 60d contents were significant differences among genotypes, 8306> H892> K326>ZY100. The content of 8306 was highest than the other three genotypes at every leaf stage. In addition to 50d leaf age , the content of ZY100 was lowest at other various ages. with growing flue-cured tobacco leaf, the three nitrogen treatment content showed a single peak discharge curve, 20d-40d increased, 40d then decreased and reached its peak, 60d reached the minimum. Among nitrogen treatments leaf surface trichome exudate content were significantly difference. Nitrogen treatment of content in each leaf stage were higher than the other two nitrogen treatmentce. Low-nitrogen treatment and high nitrogen content was not significant difference in most of the leaf age leaf secretion.After spraying SA, the overall content of leaf surface trichome exudate of three genotypes(8306,H892,中烟100) of secretion showed the same single peak, 0d-6d content increased, 6d peaked, 6d-15d decreased. 0d-3d spraying treatment were lower than CK, at 3d spraying treatment was significantly lower than CK,at 3d-6d, spraying treatment levels were higher than CK, at 6d, the spraying treatment was significantly higher than CK. But at 15d, in addition to 8306, the other two species of spraying treatment were not significant higher CK.4 In 8306 tobacco leaf total neutral aroma substances, degradation products of phenylalanine class, solanone, carotenoid degradation products, Maillard reaction products, neophytadiene were higher than K326, particularly solanone content was 41.16% higher than the K326. With the increase of the nitrogen level, 8306's total neutra material aromatic content increased, while the K326 was contrary to 8306.5 Significant analysis of petroleum ether extracts of the changes showde that the same genotype of petroleum ether extract was significantly difference among different nitrogen content, high N treatment was significantly higher than the low N treatment; petroleum ether extract content were significant differences among genotypes ,in the level of the whole, from high N treatment to low N treatment for the 8306> H892> K326> ZY100. 8306's content was significantly higher than the other three genotypes, ZY100 was significantly lower than the other three genotypes.All genotype's spraying treatment content slightly higher than CK, but the difference was not significant. Petroleum ether extract was significantly different levels among genotypes, from high N treatment to low N treatment for the 8306> H892>ZY100.6 Total nitrogen content in general showed a single peak curve, 10d-30d increased, 30d reached the maximum, 30d-40d decreased sharply, after 40d the slow decline, 60d decreased to minimum. With the increased of nitrogen, total nitrogen content of leaves was increased, total nitrogen of high nitrogen treatment was significantly higher than the low nitrogen treatment.Total nitrogen content of different genotype was significantly differences, 10d-30d increased rate of the 8306 is the smallest in the four genotypes, 30d peak at a medium level of total nitrogen content, after 30d it was the smallest declined rate, at 60d total nitrogen content was significantly higher than the other three genotypes. H892 and 8306 changes in the opposite. At 60d the total nitrogen content and nitrogen consumption senescence had a greater correlation. The different genotypes had differect nitrogen metabolism and consumed characteristics, control measures was Formulated according to the characteristics of nitrogen utilization.