The Protective Effect Of The Nicorandil Blood Cardioplegia On The Myocardial Cytoskeleton

The Protective Effect of the Nicorandil BloodCardioplegia on the Myocardial Cytoskeleton(Abstract)Aim. This study was designed to evaluate the protective effect of the adenosine-tnphosphate-sensitive potassium channel opener nicorandil as an adjunct in blood cardioplegia on the myocardial cytoskeletonMethod. Using a blood-perfused, parabiotic, Langendorff rabbit model, hearts underwent 60 minutes of normothermic ischemia protected with blood cardioplegia (St Thomas' solution (n = 8) or Krebs-Henseleit solution with nicorandil [100 i-i moIIL, n = 8]) and 60 minutes of reperftision Percent recovery of developed pressure, mechanical arrest, electncal arrest, reperfusion ventricular fibrillation, percent tissue water, and myocardial oxygen consumption were compared Left ventricular samples were examined by immunohistochemistry and Western blot using monoclonal antibodies against actin, desmin and tubulinResults. No difference in the percent recovery of developed pressure was noticed between the groups Nicorandil cardioplegia was associated with prolonged electrical and mechanical activity as compared with hyperkalemic cardioplegia (p <0 05) Nicorandil cardioplegia was also associated with a higher reperfusion myocardial oxygen consumption (p < 0 05) Ischemic tissue showed deranged pattern of actin, desmin and tubulin By Western blot bands of proteins were showed thinner after ischemialreperflision The changes of the cytoskeleton were more severe in hyperkalemic cardioplegia group than those of the nicorandil cohortConclusions. The protection of the nicorandil blood cardioplegia on the myocardial cytoskeleton is more effective than the hyperkalemic cardioplegia...