Nf-Κb P65 Expression Of The Myocardium With Different Pressure Cardioplegia Perfusion In Rat Hearts

Objective:To study the NF-κB p65 expression of the myocardium with different pressure cardioplegia perfusion in rat hearts in order to find the new way for myocardial protection on cardiopulmonary bypass.Methods:Thirty of Sprague Dawley rats were divided into three groups randomly: the control group, low pressure group and high pressure group. Control group: the normal myocardial samples were collected for study. Low pressure group : the hearts were perfused with a 4℃St.Thomas'cardioplegia solution at the pressure of 50mmHg for 5 minutes, then the myocardial samples were collected for study. High pressure group: the hearts were perfused with a 4℃St.Thomas'cardioplegia solution at the pressure of 50mmHg for 5 minutes, then the myocardial samples were collected for study. The NF-κB p65 expression of the myocardium were measured respectively by immunohistochemical staining and Western blotting. Results:Integrated optical density (IOD) was measured by immunohistochemical. Control group: there was some expression of NF-κB p65(80.1±9.10). High pressure group: the expression of NF-κB p65 was observed in the myocardium(226.7±12.5 ) . Low pressure group: the expression of NF-κB p65 was observed in the myocardium too(117.5±15.5), but the density level was obviously lower than high pressure group(p<0.05). The relative optical density was detected by Western blot analysis. Control group: there was some expression of NF-κB p65(0.44±0.05). High pressure group: the expression of NF-κB p65 was observed in the myocardium(1.58±0.13 ) . Low pressure group: the expression of NF-κB p65 was observed in the myocardium too(0.95±0.05), it was significantly lower than high pressure group(p<0.05).Conclusion:The results suggest that there were different degree expression of NF-κB p65 with different pressure cardioplegia perfusion, but the low pressure cardioplegia perfusion was better than high pressure in myocardial protection.