Histopathological Study Of The Human Lymph Node Sinus With Blood

Objective The purpose of this study was to find out the histopathological characteristics and the significances when blood present in the human lymph sinus.Materials and Methods When routine autopsy was carried out in the randomly selected 102 patients among which 100 patients died of various diseases, and 2 patients died of non-diseased reason, superficial lymph nodes located in the bilateral neck, axilla, inguina, and thorex, abdomen were sampled. Haematoxylin-Eosin staining were performed on 10% formalin-fixed and paraffin-embedded 5um cut lymph node tissue sections. The histological pattern of the lymph sinuses containing the blood were observed under the light microscope. The expression of CD31 a marker for the endothelial cell was detected in various blood containing lymph sinuses and non-blood containing lymph sinuses with the method of immunohistochemistry.Results Among the 1322 lymph nodes sampled from the 100 autopsy diseased cases, blood containing lymph sinuses were found in 809 lymph nodes sampled from 91 cases, whereas such sinuses couldn' t be seen in the lymph nodes sampled from the non-diseased cases. According to the histology, we divided the blood containing lymph sinuses into five categories: vascular-opening sinus, blood-deficient sinus, erythrophago-sinus, blood-abundant sinus, vascular- formative sinus. Immunohistochemistry findings showed that the expression of CD31 was strong positive in vascular-formative sinus and some vascular-opening sinus while it was faint in blood-deficient sinus, erythrophago-sinus and some vascu1ar--opening sinus. It was almost negative in blood--abundant sinus andnon--b1ood containing sinus. The differences of distribution of b1oodcontaining lpph sinus and its variant in different part were remarkable(p<0. 01). B1ood containing 1y'InPh sinus could most possible be found in thenode 1ocated in the neck and the auxil1a whi1e the least located places wereinguina and thorex. A11 kinds of the b1ood containing lyInPh sinuses could easilybe found in the neck. Vascu1ar sinus and vascular opening sinus were frequent1yfound in the same node. Blood--abundant sinus could rare1y be found in theinguinal 1roh node. B1ood containing 1pph sinus and the variants wereindependent of the courses of disease, the 1esion of tumor or non--tumor, thepresence of serious inf1aJnmation and cardiac fai1ure (P)0. 05).dric1usions In the state of disease, the phenomenon of blood present in the1yIIlPh sinus is not unconunn. Blood could possib1e enter the 1roh sinusthrough the afferent lrwhatics and/or the lrohaticovenous conunnicationsbetween the veins and the sinuses in the node. Lpph circu1ation and the bloodcircu1ation could communicate with each other in the 1pph sinus. B1oodcontaining lpph sinus cou1d regulate the circulation in the node indirectly.Through the blood containing lyInPh sinus, more antigen and stimu1ator care fromthe blood circu1ation could stimu1ate the lyInPh tissue and inspire theimmuno--reactions. Moreover, the skipping metastasis of ma1ignant tumor mayhave some connection with the b1ood containing lymPh sinus. In--depth researchesare needed to disc1ose more about the c1inica1 patho1ogy of the changes.