An Epidemiological Study Of Risk Factors On Male Infertility

Object i ye To study risk factors of male infertility and to investigate causes of oligospermia and azoospermia. Methods A case control study was conducted. ?The subjects include 165 male infertility patients and 50 normal controls with a bearing capability in the same region. A health examination and a regular test of semen were made. For each subject, a standard questionnaire was used to survey the medical history and potential risk factors. The correlation between potential risk factors and male infertility was analyzed by both a monofactorial and a multifactorial logistic regression. ?An enzyme-linked immunosorbent assay was used to test the FSH, LH, PRL, E2, and T value of the reproductive hormone in the serum Different groups were divided according to a normal reference defined in our laboratory. The tested values of FSH, LH, PRL, E2, and T were compared with the normal reference. The calculation results were then recorded into new values. An indicator variable was used The correlation between these indexes of reproductive hormone and male infertility was analyzed by both a monofactorial and a multifactorial logistic regression. A detailed analysis of spermatogenic cells?chromosome was conducted. The subjects include 25 oligospermia patients, 25 azoospermia patients, and 6 normal controls All the subjects were selected from the subjects in the first study. Chromosomes of spermatogenic cells were prepared by a direct low osmotic pressure method Resu Its 麯 The monofactorial analysis revealed that smoking, excessive drinking, 3 ~4~4~i;. high temperature, and semen abnormality are strongly correlated with male infertility. The mutlifactorial analysis revealed that excessive drinking, high temperature are strongly correlated with male infertility. ?The high-value of FSH, low-value of FSH, high-value of PRL, high-value of E2 and low-value of I were strongly correlated with male infertility. ?Different fission phases of spermatogenic cell can be observed in all controls and all patients of oligospermia. Out of 25 azoospermia patients, fission phases can only be observed from 15 patients. Comparing the distribution of spermatogenic cells?abnormal fission phases among these three groups, the difference is significance (P<0 0001). ~ The spermatogenic arrest in oligospermia happens partly at the first meiotic prophase. Most are in zygotene and pachytene. The spermatogenic arrest in azoospermia happens at the early stage of spermatogonia Most are spermatogonia. Cono I us i on GD Smoking and excessive drinking and high temperature are identified as risk factors of male infertility. ~The reproductive hormone abnormalities and semen abnormalities have strong correlation with male infertility. ? Chromosomes of spermatogenic cells prepared by a direct low osmotic pressure method can provide a valuable cell genetic information about male infertility and the function of the spermatogenesis in the testis. GD The spermatogenic arrest is the cause of oligospermia and azoospermia. The synapsis disappearance in azoospermia is one of the main causes that lead to the spermatogenic arrest.