Relationship Between EBV Infection,Expressions Of P53 & P16 And Non Hodgkin's Lymphoma In The Nasal Cavity And Pharyngeal Waldeyer's Ring

Objective:(a) To observe the presence of Epstein-Barr virus(EBV) and expressions of p53 and p16 in non-Hodgkin's lymphoma (NHL) of the nasal cavity and pharyngeal \Valdeyer's ring; (b) To investigate the correlation between them. Methods: In situ hybridization was used to detect EBV-DNA and p16-mRNA and immunohistochemical staining was used to examine the expressions of CD45RO, CD2O,CD56,LMP1,p53 and pl6 in 36 cases. In situ PCR was used to re-detect EBV-DNA in 5 cases whose EBV-DNA was negative by in situ hybridization. Results: Positive expression rates of CD45RO, CD56, CD2O, LMP1, EBV-DNA, p53, p16 and p16mRNA were 52. 8%, 22. 2%, 47. 2%, 27. 8%, 61. 1%, 19. 4%, 22. 2% and3O. 6%. EBV-DNA in one case was positive by in situ PCR. Positive expression rates of EBV-DNA in T-NHL were significantly higher than in B-NHL( 7 PO. 05).Conclusion:A higher percentage of EBV infection was present in NHL of the nasal cavity and pharyngeal Waldeyer's ring, especially in T/NK-NHL.EBV infection was not significantly associated with p53 and p16 expressions in NHL.