| Perfluorocarbon(PFC) is one of blood substitutes,and has the capability that carries high oxygen,exclude carbon dioxide.lt is man-made chemical compounding of nonpoisonous and non-blood elements in it.In 1966,Clark prove that Perfluorocarbon emulsion possesses carrying the oxygen ability.The researchs has been in progress to Perfluorocarbon in nations such as the U.S.A , Japan , France and Germany etc , and some progress have been gained.In 1972, Japan carried on the normal human body experiment, and the clinical experiment of PFC is carried on in Japan and U.S.A in 1979.But in this process of study and the application ,the fundamental research is clear inadequately , and the effectiveness and safety of PFC need to get the experiment certification further;in the course of heavier degree hemorrhagic shock, brain tissue damaged by short of the cerebral blood and short of oxygen supplying, and in the treatment to hemorrhagic shock of PFC, the protection effect to brain tissue is not effectively confirmed in experiment . This is the main propose of the experiment.This experiment use the new second generation carrying oxygen and nanometer type PFC, and this type PFC is composed by using the special type light source to carry on the actinic reaction , and choosing specially designated catalyzer and high efficiency refinement technology to separate and purify , and the outgrowth and raw material that does not react except clean ,the purity degree 99.5%. Uses the nanometertechnology prepare the preparation , and gets the average granul diametre to control for 100 nanometers and even stable distributes latex. PFC possesses good chemistry stability and hot stability. This PFCcarries oxygen ability 40-50ml%, about serve as the water 20 times, 2 times of blood. All not being destroyed in the acid and alkalescence solution , the change does not also occur in the heating . This preparation can stablely be stored under normal temperature,and can be exterminated bacterium under the high temperature and high pressure, and the peculiarity is not possessed in old tyoe PFC.Objective:Establish rabbits model to observe and view the function of effectiveness and safety of PFC in the course of treatment to hemorrhagic shock ,and the function of protecting cerebral cortex nerve cells.Methods:A totle 20 Male New Zealand rabbits in 2 groups(each of the 2 groups consisting of 10 rabbits).All of rabbits are completed the modle of hemorrhagic shock .Experimental group used PFC and control group used 706 blood substitute.In the course of treatment,all of rabbits breath oxygen in veil.In the course of experiment,heart beat ,breath rate,blood pressure,artery vigour,pupil, light reflect,survival rate in 12hours are observed.To every sample,in the same parietal lobe cortex,with electron microscope and light microscope stucture is observed.Date were reported as mean + SD. Statistical significance levelswere calculated by using Analysis of t test and x test.Results:In 12 hours survival rate in experimental group is higher than control group.The heart beat rate is not significantly different in bothgroup.Breath in experimental group is deeper than control group and berath rate is also faster.In experimental group,blood pressure and PaC>2 is significantly higher than it in control group. PaCC>2 in experimental group evacuate higher than central group.The control group has the trand of going acid.Pupil light reflect in experimental group is sensitiver than control group. From light microscrope the parietal lobe cortex in experimental group are damaged less than control group,the structure and shape are almost normal, nucleolus clear;the neuron in control group swell,nucleolus extinct,central Nissl body extinct.The number of damage neuron is significant different in both group. From electron microscope in control group nuclear chromatin in neuron is rare,nuclear membrane is not clear,mitochondria ribosome swell,mitochondrial crista extinct,ribosomal aggregate,structure in neuron disorder and extinct.There are not ab...
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