Study Of CD23 And CD19 Expression On Peripheral Blood Lymphocytes In Asthmatic Children

Bronchial asthma is a disease characterized by elevated level of IgE and eosinophilic inflammation of the airways and airway hyperresponsiveness. Activation of antigen-specific T helper(Th) 2 cells in the lung with subsequent release of interleukin(IL) 4 and IL-5 is believed to play an important role in the pathogenesis of asthma. The pathologic basis of asthma is chronic airway inflammation. The expression of CD23 and CD 19 on lymphocytes may be associated with this chronic airway inflammation.Objective To explore the role of CD23 and CD 19 in pathogenesis of childhood asthma and the relationship between CD23> CD 19 and severity of asthma and the relationship between CD23 and CD 19.Method 30 asthmatic children hi attack and 30 asthmatic children in stable stage were enrolled and 30 healthy children were collected as control. The percentage of CD23+ lymphocytes and CD 19+ lymphocytes and CD23+CD19+ lymphocytes in peripheral blood were measured by flow cytometry. The serum level of IgE were measured by ELISA.Results The results of determinations are as follows: (l)The percentageof CD23+ and CD19+ and CD23+CD19+ lymphocytes in peripheral blood: asthmatic children in attack were, respectively^8.13?3.23)?0.05). (2) The serum level of IgE(IU/ml) were respectively: asthmatic children in attack (484.33 + 375.75 X asthmatic children in stable stage (345.97?20.66) and healthy control children (135.24?7.21) .There were significant difference (PO.05) within these three groups (3).In attack stage, the percentage of CD23+ and CD19+ and CD23+CD19+ lymphocytes cells in peripheral blood(%) were significantly increased in moderate/severe asthmatic children (10.13 + 2.86, 27.11 ?.67 and 9.63?.81, respectively) in comparison with mild asthmatic children (6.80?.80, 19.52?.74 and 6.40?.74, respectively) (p<0.01,). (4) In attack stage of asthma, the percentage of CD23+ lymphocytes correlated positively with the percentage of CD19+ lymphocytes (r=0.698),and the percentage of CD23+CD19+ lymphocytes (r=0.588).Conclusion (l)Expression of CD23 and CD 19 on lymphocytes hi asthmatic children were significantly increased. In attack stage they were much higher than in stable stage and the percentage of CD23* lymphocytes positively correlated with percentages of CD19+ and CD23+CD19+ lymphocytes. (2)In stable stage of asthma, expression of CD23 and CD 19 on lymphocytes washigher than healthy control children.This provide evidence that asthmatic children need lasting anti- inflammation therapy. (3) In attack stage of asthma,expression of CD23+ and CD19+ and CD23+CD19+ lymphocytes was higher in moderate/severe asthmatic children than in mild ones.