Expressive Differentiation Of TGFβ1 In Bladder Transitional Epithelial Cancers And Its Regulation To MMP2 And Angiogenesis

Bladder transitional epithelial cancer is the most common neoplasm to urologist in China. Their incidence rate has improved in recent years. Degradation of the extracellular matrix (ECM) and basement membrane is believed to be associated with tumor invasion and metastasis. Angiogenesis is also essential for tumor growth and metastasis. Transforming growth factor ?1 (TGF Pi) is a multifunctional polypeptide that influence cell growth and differentiation, cell adhesion and migration, extracellular matrix formation, angiogenesis and immune function. In present study, we investigated the expressive differentiation of TGF 3 1, MMP2, TIMP2, factor Vffl and VEGF in specimens of patients with bladder transitional epithelial cancer.The expression of TGF P 1, MMP2, TIMP2, factor Vffl and VEGF were determined in 42 bladder transitional cell carcinomas and 6 normal bladder mucosas by immunohistochemistry.The positive value of TGF P 1 in bladder transitional epithelial cancer is 64%. TGF P 1 expression in invasive and G3 tumors is higher than superficial and Gl,2 tumors, but this was not statistically significant. The positive value of MMP2, TIMP2 and VEGF in bladder transitional epithelial cancer is 83%, 58% and 74% respectively. The expression of MMP2 and VEGF is significantcorrelated with tumor stage and grade, however, this correlation is not found between TIMP2 expression and tumor stage or grade. The expression of MMP2 and TIMP2 has no correlation. The invasive and G3 tumors have significant higher MVD than superficial and Gl,2 tumors. TGF P 1 expression is significant positive correlated with MMP2 and VEGF expression. The MVD in tumors which were both TGF J3 1 positive and VEGF positive were the highest of all the subgroups.These results suggest that degradation of the extracellular matrix by MMP2 and tumor angiogenesis are essential for bladder cancer, especially in invasive and high grade tumors. TGF P 1 might be associated with tumor invasive and progression by up-regulation the expression of MMP2 to degrade the extracellular matrix and also by indirectly stimulating angiogenesis through the up-regulation of VEGF expression in bladder cancers.