| Hemoperfusion, precisely the blood adsorption, is one of the important conformations of blood purification. In order to remising the clinical symptoms, healing the disease, reducing the mortality rate, blood draw assistance from extracorporeal circulation, passing the perfusion adsorbent column, which obtained the effective detoxification or specific ligand to cleaning the sickness substances. Hemoperfusion has the wider subject range, lower cost price, mainly be used in rescue the acute or severity intoxication by drugs or poisons; acute or chronic liver function failure; acute or chronic renal failure; thyroid crisis; disease of immune system; schizophrenia; psoriasis et al.The commonly used adsorbent of hemoperfusion is mainly the activated charcoal and various kinds of resin. In different degrees, they all exist the problems, such as poor adsorption selectivity, different blood compatibility, and unstable blood kinetics in hemoperfusion process. It's far from gratifying the clinical therapy's requirement.Investigating and developing a new type of adsorbent with higher efficiency, higher selectivity, realizing the perfusion column miniaturization, difference is the important topic in blood purification's investigation.The specific effects and adsorptive behavior that the nanometer materials possessed are not the common materials had. To investigate their adsorption effects can try to find out their adsorption mechanisms and succeed to develop some hemoperfusion's materials, which have higher effective; and otherwise, it can suggest a new request for nanometer materials' synthesis produce and expand their application area. This investigation will have important value of theory and application.This article utilize the carbon nanotube and montmorillonite as adsorbent, use sodium Phenobarbital and chlorpromazine hydrochloride as model compounds, compare with the frequently hemoperfusion's material桱apanese conglobate actived carbon, in order to investigate nanometer materials' adsorption behavior in vitro, and then verify the adsorption result by in vivo experiments. Materials and Methods? Recommend of the Carbon nanotubeThe Carbon nanotube has fantastic physical or chemistry capabilities, such as regular vacancy st^ucture^ can be controled to growing - has more vivid reactivity and stronger selectivity,so it has been made as the ideal model of adsorption performance and has the important value of academic study.? Recommend of the Nano-MontmorilloniteMontmorillonite is the typical uni-dimension nanometermineral, provides the 2:1 form expanding structural of moisture Aluminium Silicate, imbalance charge inside, a great deal of configurations in exterior, so it can be reshaped and developed in order to have finer adsorption behavior.H. Experiment materials(?) . Adsorbent: Globular activated carbon (AC); Carbon nanotube (NO; Nano-Montmorillonite (MMT) (Zl) . Poison: Sodium Phenobarbital;Chlorpromazine bydrochloride(H) . Species of Kunming mouse and plasm of health rabbit ( 0]) . Instrument and Reagent:UV spectrophotometer ^ Sedimentator > OscillatOx Pipe; Dichloromethane^ Sodium hydroxid^ Amchlo^ Hydrochloric acid, et al.0. Experiment methods (梸) Adsorption1. Analysis of poisonSpectrophotography2. Static adsorption testsAdding different Concentration's poison plasm solutions in AC^ NC > MMT, homoiothermy balneum tepidum oscillation, then hypothermia Centrifugation, measure the Concentration of the rest poison.3. Data processingCalculate the adsorption dose and the adsorption rate.Adsorption equilibrium data use "the Langmuir equation" and "the Freudlich equation", obtain the parametes?Statistics: Use linear regression analysis of variance by SPSS 10.0 for windows ( ? Animal experiments in vivo1. Choice of Mouse and divide into groups2. Define the Concentration of adsorbent and poisonMouse' Minimus lethal dose of Sodium Phenobarbital is 25mg/ml; Mouse' liminal va...
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