The Significance Of The HVE DNA Detection Of The Adjacent Liver Tissues In The HCC To The Forcast Of The Recurrence After Hepatectomy

Objective To observe the effect of HBV-DNA in the recurrence of hepatocellular carcinoma (HCC).Methods Polymerase Chain Reaction (PCR) was used to detect and autographic HBV-DNA in fresh peripheral blood, tumor and their adjacent liver tissues of the HCC patients. We contrasted our result with that of following observation after hepatectomy and analysed the results by demographic method.Result In 33 patients, 87. 9%(29/33) with HBV history, positive detecting rate of HBV-DNA in peripheral blood was 57. 6%(19/33). In HCC tumor tissues the positive detecting rate of autographic HBV-DNA was 9. l%(3/33), integrated HBV-DNA was 78. 8. l//o(26/33). In adjacent liver tissues of the HCC the positive detecting rate of autographic HBV-DNA was 81. 8% (27/33), integrated HBV-DNA was 75. 8% (25/ 33). The recurrent rate in serum HBVDNA positve group was 63. 2%(12/19) , in negative group was 57. l//o(8/14), there were not statistically significant (P>0. 05). The recurrent rate in tumor tissue autographic HBVDNA positve group was 66. 7% (2/3) , in negative group was 60. 0%(18/30), there were not statistically significant (P>0. 05). The recurrent rate in tumor tissue integrated HBVDNA positve group was 73. 0%(19/ 26) , in negative group was 14. 3%(l/7) , there were statistically significant (P<0. 01). The recurrent rate in adjacent tumor tissue autographic HBVDNA positve group was 70. 4%(19/27), in negative group was 16. 7%(l/6), there were statistically significant (P<0. 05). The recurrent rate in tumor tissue integrated HBVDNA positve group was 64. 0%(16/25) , in negative group was 50. 0%(4/8), there were not statistically significant (P>0. 05). The results reveal most HCC patients have HBV history, most patients have autotype HBVDNA in tumor and adjacent tissues, HBVDNA autotype was found in adjacent tumor tissue. The recurrence of HCC was closely related to the HB-VDNA integration in tumor tissue and autotype in adjacent tumor tissue, there was no related to the HBVDNA quantity in blood serum. The tumor size was closely related to the tumor recurrence, The recurrent rate in group with tumor calibar less 3 cm was significantly lower than that in group with tumor calibar over 5 cm (There were 73. 3% and 20.0%, P<0. 05).Conclusion Most HCC was related to HBV chronic infection, The recurrence of HCC was closely related to the HBVDNA integration in tumor tissue and HBVDNA autotype in adjacent minor tissue. Detection in HBVDNA integration of tumor tissue and HBVDNA autotype in adjacent tumor tissue was a significant indication to find the tumor recurrence early after liver resection.