The Role Of Boschniakia Rossia Ethanolic Extract Treatment In Dimethylnitrosamine-induced Rat Liver Fibrosis In Vivo

Liver fibrosis is the critical stage of cirrhosis induced by various chronic liver diseases ,meanwhile ,this stage is the most effective therapy in the development of chronic hepatic injury .During the past 10 years ,many researches demonstrated that Chinese medicine have effects on liver protection and anti-fibrosis. To understand the role of Boschniakia Rossia ( BR) in treatment of liver fibrosis ,we carry out present experiment.Methods: we investigated the role of BR in a model of liver fibrosis induced by dimethylnitrosamine(DMN) intraperitoneal injection. When the model has been made , the rats were given BR ethanolic extract with different dosage intragastrically, and they were compared with Colchicine protective group to observe the treatment effects. At the end of the experiment , the rats were anesthetized and blood samples were adopted from hearts. The activity of malondialdehyde (MDA) ,Superroxide Dismutase (SOD) and Glutathione peroxidase (GSH-PX) in serum and the concentration of serum Hyaluronic Acid (HA),Procollagen Type â…¢( PCâ…¢) and Type â…£ Colagen (â…£.C) are detected .On the other hand ,liver tissues were adopted immediately after the rats were killed. We also examine the activity of MDA,SOD and GSH-PX in liver tissues. The morphological changes of liver tissues were evaluated by pathological examination.Results: 1 serum markers1.1 higher HA,PCâ…¢ and â…£.C consentration in model group are significantly than those in normal group ( p<0.01). while HA,PCâ…¢ and â…£.C in normal-dose and high-dose of BR groups and Colchicine group were much lower than those in model group ( p<0.05). 1.2 SOD and GSH-PX activity of serum in model group are significantly lower than those in normal group ( p<0.01). while MDA concentration of serum in model group is significantly higher than normal group. SOD and MDA in normal-dose and high-dose of BR groups are observed that have significantly differentiation than those in normal group ( p<0.01). However , MDA,SOD and GSH-PX of Colchicine group have no differentiation with normal group ( p>0.05). 2 Liver tissue target: the activity of SOD and GSH-PX in model group have significantly lower than those of normal group ( p<0.01). But the concentration of MDA in model group have reverse result with normal group ( p<0.01). while the activity of SOD and GSH-PX in normal-dose and high-dose BR group have significantly higher than those of model group ( p<0.01). In the same way , the concentration of MDA in normal-dose and high-dose BR group havereverse result with model group .The result of SOD ,GSH-PX and MDA between Colchicine group and model group is same as those in serum.3 Pathological examinationthere are extensive hepatocyte degenerate and necrosis in model group .In Sirius Red staining segment ,we can find extensive fibrous tissue proliferation and the presence of regenerative parenchymal nodules, normal lobules were destroyed. Regenerative nodules may contain hepatocytes from one or more than one lobule and are separated from adjacent nodules by fibrous tissue, the degree of fibrosis is more severe than normal group , and have significantly differentiation ( p<0.01). the degree of fibrosis in Colchicine group ,normal-dose and high-dose BR group are significantly lower than those in model group ( p<0.01).Conclusion:These findings indicate BR has profound perspective in the therapy of DMN-induced rat liver fibrosis in vivo.