Study On The Correlation Between SCT Features,Pathology,MVD,Expressions Of VEGF,TGF-β1 In Gastric Carcinoma

Background and purpose f The examination about gastric cancerusing SCT is perfOrmed successfully through spiral scanning,automatic power injector and water-filling method. Using triphasicdynamic contrast-enhanced SCT evaluating gastric cancer hasbecome more and more mature. Gastric cancer is divided into tWosubtypes by Lauren in l965; intestinal type and diffose mpe. The tWosubtypes are different in epidemiology. pathology. biologic actionand prognosis. Therefore, they have different medical imagingand it is important to make sure of the exact type of gastric cancerbefore operation using medical imaging examination. In recent years,the angiogenesis of gastric cancer has become one of hotspotS of5$Nk7 20o2 $5HA$itX Wj@$m CT W$5mg. MVD & VEGF. TGF-nl &&(edX#toWAstudy. Vascular endothelial growth factOr (VEGF) and transfOrminggrowth factor-betal (TGF- fl l) are correlated with angiogenesis.Microvessel density (MVD) can be an indictation of measuring thenew vesse1s in gastric cancer The pUrpose of mis study is to try toevaluated the correlation betWeen SCT featUres \ Lauren'sclassification. MVD. and expressions of VEGF. TGF- D l in gaStriccarcinoma.Objects and mcthods; Both plain SCT scan and triphasicdynamic contrast-enhanced SCT scan were performed in 40 patientswith advanced gastric cancer (cardiac carcinoma 15, body carcinoma7, antral carcinoma l6 and leather stomach 2). The scbogparameters of SCT are l20KVp, 280tnAs, collAnation 5mm, pitChlf l. The contrast medium was Omnipaque ofl00ml(300mg.I/ml),the injection speed was 3mUsec. 'Ihe scanningimaging of the arterial phase, the venous phase and the equilibriumphase was obtained at 25s. 5s. 2-3min after the beginning of theinjection of contrast medium, respectively All 40 patients undenventtotal. subtotal gastrectomy and exploration laparotomy with a weekafter SCT sctring. After operation, the specimens were sent to thedepartment of pathology, where they were embedded witi1 paraffmroutinely. Five-micrometer-thick sections were obtained from theparadrirembedded specimens. Detection procedure fOr VEGF'6nWX$2002 $ffntit1 W$$$CT &$5teN. MVD & VEGF. TGF-81 $8f NXFMWAMVD' TGF- 6 l were perfOrmed using inununohischendstryStatistical analysis was executed by SPSS l0.0 software. Thenumeration data of the groups were compared using t-test, the binmpdare were comprared using Fisher's exact test. T'he P values less than0.05 were considered sighficanly different.Rcsults: (l) In 40 patieflts,VEGF exPression was detected in l6patients, The VEGF9ositive rates were 40.00% (l6/40). TheVEGF-positive rates were 85.7l%(12/l4) and l5.38%(4/26) inpatitents with and without lymph node metastases, and the differencewas statistically significant(P<0.05). The VEGF-positive rates were83.33%(5/6) and 32.33% (ll/34) in patitents with and without livermetaStases, and the difference was statistically significan(P<0.05).(2)hi 40 patientS, TGF- 6 l exPression was detected in l2 tumors. TheTGF- 6 l-positive rates were 30.00%(12/40). The TGF- fl l-positiverates were 42.3l%(ll/26). 7.69%(l/l3) in patients with and withoutserosal invasion respectively, and the difference was statisticallysignifican(P<0.05). The TGF- D l-positive rates were 80.00% (4/5 )and 22.86%(8/35) in patients with and without peritoneal metastasesrespectively, and the difference was statistically significant(P<0.05).(3) Microvessel densities ranged from 4.8 to 49 with a mean of26.04(standard deviation, ll.96). The microvessel densities were30.9l l l2.22 in VEGF positive tUmors and 22.78t l0.83 in VEGF7$Wk$2002tyWfttitZ W$$$CT tl%5fkg. MVD R VEGF. TGF-8l $isfedk$ghWnnegtive tumors, and the difference was statisticallysignificant(P<0.05).The microvessel densities were 29.80i l2.43 inTGF- 6 1 positive tUmors and 24.42i 1l.60 in TGF- fl l negtivetumors. Although there was a tendency fOr higher microvessel in the.group with TGF- D 1 posi...