| To study the relation of time-effect of accumulated electroacupucture(EA) on focal cerebral ischemia reperfusion injury to rats.Methods:1. Using the two-kidney, two clip method to produce the stroke-prone renovascular hypertensive rats(RHRSP), then using a suture-occluded method to induce the model of focal cerebral ischemia reperfusion injury.2. Acupuncturing 24 hours after the MCAO model was made. Qd, 6 days/ per course.3. Based assessment: evaluation of neurography grade, histopathological changes of brain tissue, determination the level of superoxide dismutase (SOD) and malondialdehyde (MDA) of brain tissue, determination of hemorheology. Results:1. There was statistic significance among pseudo-operation controlled group, ischemia reperfusion group, group of retaining needle for 20 minutes and group of retaining needle for 60 minutes (/?<0.0001) .2. There was remarkable ischemic brain damage occurring in the embolismic cortex of frontal lobe, parietal lobe and temporal lobe.3. Contrasting with group of retaining needle for 20 minutes, group of retaining needle for 60 minutes, there were the decrease of MDA level, the increase of SOD level and the improvement of hemorheology condition (p<0.05) .Conclusion:Contrasting with group of retaining needle for 20 minutes, group of retaining needle for 60 minutes has obviously decreased neurography grade and MDA level, increased SOD level and improved hemorheology condition. The results showed that in the acupuncture treatment of focal cerebral ischemia reperfusion injury to rats, the effect was obvious and related with the time of retaining needle. The effect of group of retaining needle for 60 minutes was better than that of group of retaining needle for 20 minutes.
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