Research On Standardization Of Quality Chemical Control Of Rhizoma Dryopteris Crassirhizomae

In order to standard crude drug quality of Rhizoma Dryopteris Crassirhizomae, quality standards were founded by systemically retrieving articles corresponding it's chemical and pharmacological research in the world, then the research about it was finished is shown following:1. Clarification of the active substance in Rhizoma Dryopteris Crassirhizomae,All the essays about its chemical and pharmacological research show that compounds of flocinon (dryocrassins) possess the effects of anti-bacterial, anti-virus of AIDS and anti-tumor. So the research of Rhizoma Dryopteris Crassirhizomae, quality standard was carried on basing on the standard of dryocrassin.2. Preparation and accumulation some quantitive standardsOptimization of preparing for high purity chemical control of dryocrassin is recrystalization, which was drawn from four kinds of screening test such as Column chromatograph, thin layer chromatography, HPLC and Recrystalization. In such way 1.8g dryocrassin with the purity of 99.81% proved by HPLC was got.3. Identification the structure of dryocrassinBy way of physical-chemical method, thin layer identification and four kinds of spectrometry桬SI-MS, infrared 3D spectrometry, IR 'H-NMR, 13C-NMR, 'H-13C cozy 2D-NMR, 13C-13C cozy 2D-NMR, and 13C-NMR is used for the first time in the identification of the compound.4. Founding the analysis method of the quality and quantity of dryocrassinAs a control of dryocrassin, the chromatography condition was founded to analysis the crude drug character and quality for the first time in the world and then some methodology studies were carried on.5. Setting up the quality standard of Rhizoma Dryopteris Crassirhizomae,A sysmatical research about Rhizoma Dryopteris Crassirhizomae was carried out, such as denomination by Pharmacopiea, identification of source plant, location , collection, characters, preparing method, identification of quality (including morphology, physical-chemical character, thin layer chromatography and HPLC), content assays (including chromatography condition and systematic adaptability test, preparation of control solution, preparation sampler solution, and analysis method) character and action, channel tropism, function of drug and main action, usage and dosage, storage etc.. Basing on these researches, the scheme and clarification about modern quality standard in Rhizoma Dryopteris Crassirhizomae were completed, among them , fingerprint diagram of HPLC, quality analysis of active component in control by HPLC have made up the shortage in Pharmacopiea.