Research On The Cellular Immunological Function Of Traditional Chinese Medicine Type Of Syndrome That Stagnation Of The Liver-Qi And Deficiency Of The Spleen And Retention Of Damp-heat In The Interior From Patients With Chronic Hepatitis B

Objective:To explore the trait on traditional Chinese medicine (TCM) type of syndrome that stagnation of the liver-Qi and deficiency of the spleen and retention of damp-heat in the interior,provide objective basis for TCM differentiation of symptoms and signs.Methods: 60 patients ,included stagnation of the liver-Qi and deficiency of the spleen 32 and retention of damp-heat in the interior 28,meanwhile, 20 cases as normal controls.T cell subsets were tested by APAAP method,serum Thl/ Th2 cytokine (1L-2,1FN- Y ,lL-10)Level were tested by ELISA method.At the same timejiver functionjiepatitis B virus(HBV),HBV-DNA,hepatic fibrosis, blood type were tested in patients.Results:(1)Numbers of CD3+, CD in stagnation of the liver-Qi and deficiency of the spleen were both lower than that in retention of damp-heat in the interior(P<0.05 ,P<0.01),and the numbers of its CD4+also lower than that in controls(P<0.05). compared to controls,numbers of CDs had not distict differences in both of the two groups.while CD, CD, ratiodecreased (P<0.01,P<0.05).Compared to retention of damp-heat in the interior, CD CD ratio decreased distinctively in stagnation of the liver-Qi and deficiency of the spleen(P<0.05).(2)Compared to controls,the levels of IL-2 and IFN- Y in both of the two groups had not distinct differences(P>0.05). Compared to controls and retention of damp-heat in the interior ,the level of IL-10 was higher in stagnation of the liver-Qi and deficiency of the spleen(P<0.05).liver function TBA had distinct difference(P<0.05),butA,G,A/G,ALT,hepatitis B virus(HBV),HBV-DNA, hepatic fibrosis(HA* HIP, LN) and blood type had not distinct difference between the two groups.Conclusion:Compared to retention of damp-heat in the interior, hepatic injury of stagnation of the liver-Qi and deficiency of the spleen was gentle.Both of them were disorder in immunological function.Ratio of CD// CDg+ decreased, Thl/ Th2 cytokine lost balance.The two groups had distint differences.lt was indicated that the differences of immunity cloud provide objective basis for TCM differentiation of symptoms and signs.Treating chronic hepatitis B according to immunity was reasonable.