| Purpose1. To study the relationship between damages on rabbit retina after laser irradiation of four different wavelengths (532, 755,810and1064nm) and damage-related factors.2. To observe and analyse the self-recovering function of the damages on rabbit retina.3. to research on biological effects of MLG-01 and MLG-02 solo-soldier laser protective eyewear.4. To starte a preliminary research on retina damage after laser irradiation and the recovering mechanism through the study of the influences on ICAM-1, IL-1β and MPO when retina being hurt by laser,.Methods1. Design light-paths of the damage of rabbit retina for laser irradiation and set up a model.2. Observe, analyse and compare the different cases of the damages of the rabbit retina after irradiation by four different wavelength laser using funduscopy, fundusphotography and tissue section of fundus including damage on the retina by different energy-density solo-pulse laser; retina damage by same, energy-density and wavelength but different pulses; and the influences on the damage when laser functioning at the point in the fundus.3. After the damage of the fundus of rabbit retina by laser, use funduscopy and fundusphotography to observe and analyse the self-recovering mechanism of the eye after 1, 3, 7, 14 days and a month.4. Let the rabbit put on laser protective eyewear to observe the maximum energy-density and power-density which the spectacle can endure, compare the degree of damage on the rabbit retina when without spectacle thus find out theprotective point of the laser protective eyewear.5. Use in situ hybridizationa to observe the changes of ICAM-1, measure the changes of MPO to weigh the changes in volume of PMN; use radio-immunity method to measure the volume changes of IL-1βand carry out a preliminary analysis of the retina damage and its recovering mechanism.Results1. When laser caused same retina damages 532nm energy-density was the weakest, 1064nm was the strongest, while 755nm was moderate. 2. Retina damages became heavier with the increase of energy-density of the laser of the same wavelength and pulse wideth.3. Retina damages became heavier with the increase of pulse of the laser though with the same wavelength and same pulse energy-density.4. In the damages caused by four different wavelength laser, bleeding of choroid in the tissue section of rabbit eye when irradiated by 1064nm wavelength, 5ns pulse-width, 1924mJ/cm2 density laser was found but no bleeding of choroid was found when irradiated by three other wavelength laser. retina to choroid. 5. In the experiments of rabbit retina bleeding by the four wavelength laser irradiation, there were different types of bleeding, some were spot-like, some were chrysanthemum-like and some were irregular block-like. 6. When the rabbit eyes was irradiated by 1064nm wavelength, 4998mJ/cm2 energy density, 5ns pulse-width, single pulse laser, 3×1mm2 wound paths was found in some rabbit lens. When the wound paths appeared, the damage of the retina decrease. When irradiated by 532nm wavelength, 756mJ/cm2 energy density, 4ns pulse-width single pulse laser, white linear mixture was found in rabbit lens. 7. Without any medical treatment, photocoagulation of rabbit retina (diameter 1/3PD) recovered in a month. Photocoagulation (diameter 1PD) beceome little white sport in a month. Photocoagulation (2-3PD) absorbed slowly, no obvious change in diameter in a month. Spot-like bleeding totally absorbed in a week. After laser irradiation, chrysanthemum-like or block-like bleeding area completely absorbed in half a month. vitreous hemorrhage beganto absorb slowly, dark red old bleeding could still be found in a month.8. MLG-01 and MLG-02 solo-soldier laser protection eyewear was sufficient to protect hare against retinal hemorrhage and vitreous hemorrhage by laser of four wave-length. 9. The first day after the injuries, the volume of MPO of retina increased slightly and the volume of IL-1β increased greatly(p<0.05); the third day after...
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