| Objective: To investigate the antioxidative activities of vitamin C, E at different levels of dosage in guinea pigs and rats.Methods: 1. 48 healthy young guinea pigs (weighed from 180g to 240g) were selected and randomized into 4 groups with 12 hi each group. Each of mem hi different group took different dose of VC (0, 7.5, 125, 250mg/kg wt) for 20 days. The second group was the control group because 7.5mg/kg wt was near to the appropriate dose for human RDA. Urine was collected hi the mid of the trial and whole blood was collected by the end of the trial. 2. 39 healthy weaning Wistar rats (weighed from 65g to 85g) were randomly allocated to 3 treatment groups with 12-14 hi each group. Those rats were fed on fully compounded diet for 2 months. The content of vitamin E hi each group diet was separately 24.18,70.83,114.04mg/kg fodder. The first group was the control group. Urine and whole blood were collected after experiment. 3. Serous vitamin C % serous vitamin E and SOD, GSH, MDA were examined; DNA damage were analyzed by SCGE; O6-Meg was analyzed by high performance capillary zone electrophoresis; erythrocyte membrane fluidity was detected by fluorescence polarization method. Results Vitamin C experiment:1. After 20 days' intervention, there were great differences hi serous vitamin C among those groups (F=107.595, p<0.001). The deficient group was 6.26Mmol/L, the control group (24.46Mmol/L) and the two supplemented groups were separately 2.91 folds, 8.36 folds, 9.30 folds higher than the deficient group.2. The difference of serous SOD among these groups had no significance (F=1.151, p>0.05). MDA of the deficient group was 2.20nmol/ml which was higher than the other three groups (0.70nmol/mLx 1.27nmol/mL% 0.87nmol/mL) while there were no differences among the three groups. GSH-Px was reduced hi the deficient group (p<0.05). But in the supplemented group, there was no significant change (p>0.05).3. The fluorescence polarization of erythrocyte membrane of the four groups were 0.3049^ 0.2817, 0.2791, 0.2770, the deficient group was the lowest and there were no differences between the supplemented groups and the control group (p>0.05).4. After the experiment, intrinsic DNA damage had no differences hi 4 groups ( F=0.453, p>0.05) , but DNA damage induced by 10Mmol/L H202 had significant differences (F=2.709, p<0.05):the control group ( 72.56 AU ) was less DNA damage than those ofother three groupsC 127.29> 121 <, 133 AU respectively, p<0.05). DNA damage induced by 25rtnol/L and 50Mmol/L H202 had no significant differences (F=0.542, 0.746, P >0.05).There were different levels O6-Meg in four groups (F=3.028, p<0.05). The level in the deficient group was higher than the control group (p<0.05). Vitamin ?experiment:1. The serous vitamin E levels were 8.64mg/L, 56.94mg/L and 64.34mg/L respectively in three groups (F=74.76, p<0.01). The values in two supplemented groups were greatly higher than the control group (6.6-fold, 7.4-fold of the control group).2. No any differences of intrinsic DNA damage in three groups were detected ( F=0.555, p>0.05). When DNA damage were induced by 5Mmol/L, 10 umol/L and 25umol/L H202, there were also no differences among those groups (p>0.05).3. O6-Meg levels in three groups were detected and had no significant differences (F=2.493, p>0.05).Conclusion1. 'Vitamin C is necessary for the growth of cavy and a high dose supplementation has no additional effects on the growth of cavy.2. Deficiency of vitamin C could lead to a reduction of GSH-Px, increasing fluorescence polarization of erythrocyte membrane and MDA.3. Different doses of vitamin C did not affect the intrinsic DNA damage. HjOi could induce DNA oxidative damage. But VC supplementation could not show the protective action.4. Supplementation of vitamin E has no effects on the growth of young rats and did not increase their serous SOD, GSH-Px and decrease MDA, fluorescence polarization of erythrocyte membrane.5. Vitamin E could not protect DNA from oxidative damage...
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