Pharmacodynamic Studies On Fu Fang Jiang Ya Pian

Abstract Fu Fang Jiang Ya Plan is comprised of the effective ingredient of Uncaria rhynchopyhlly and Apoacynum venetun L. Uncaria rhynchopyhlla is Rubiaceae plant and distributed mainly tropical regions, which has long applied history in china and is used usually to treating cardiovascular and nervous system diseases, such as light-headedness,convulsion,numbness,hypertension, etc. Apoacynum venetum L. is Apocynaceae plant. Its root has diuretic effect; the leaf can decrease blood pressure and enhance immunologic function. Furthermore, Its decoction has the action of sedation and analgesia.In our experiment, we studied the effects of Fu Fang Jiang Ya Pian on blood pressure and the symptom relevant to hypertension. Rhychoplline (Rhy) is a major effective ingredient extracted from Uncaria rhynchopyhlly ,80mg/kg of which can decrease obviously ANR's blood pressure by the means of injecting through duodenum . Furthermore, the ratio of Rhy to the effective ingredient of Apocynum 1:4 1: 2 1: 1 is given separately through duodenum to the ANR, each dose of them can decrease the ANR's blood pressure, however, the ratio of Rhy to Apocynum 1:2 is better and more stable than the other doses. Therefore, in Fu Fang Jiang Ya Pian, we decide that the optimal dose of po. is 240mg/kg, namely Rhy is 80mg/kg, the effective ingredient of Apocynum is 160mg/kg.We duplicated the model of 2K1C-RHR, the results indicate that after operation the rats' blood pressure are elevated obviously than before operation, the range is approximately 30mmhg .It means the model of hypertension is successful. 240mg/kg 120mg/kg 60mg/kg were given po. for 10 days, RHR' blood pressure can be obviously decreased (P<0.05-0.001). At the end of the continuous given 10th day, the blood pressure decrease to the minimum, the maximal range of decreasing blood pressure is 22.24%. Meanwhile, we assay the content of Angll and ANP, the results indicate that Fu Fang Jiang Ya Pian can increase the content of ANP, but the effectisn't obvious; the changing of ANP is little.In SHR, Fu Fang Jiang Ya Pian(24(K 120 60mg/kg) were given po. for 20 days, the increasing of blood pressure of SHR was inhibited obviously from the third day (P<0.05). At the end of the 20th day, SHR' blood pressure decreased to the minimum, the maximal range wasl8.95%. Moreover, AngII ANP ET were measured. The result showed the content of ANP between test and control groups was very obvious, this may be one of the mechanisms of decreasing blood pressure. However, AngII ET have no change compare with control group.In guinea pig's vertigo model, Fu Fang Jiang Ya Pian can relief the dazzle symptom of guinea pig, lessen significantly eyeball tremor and head shaking numbers caused by dripping chloroform from guinea pigs' ears.In the meanwhile, we observed the effect of Fu Fang Jiang Ya Pian on rats' blood rheology .The results suggested that the tested medicine can decrease obviously the whole viscosity blood sedimentation. The difference between the test and the control groups was very notable. Moreover, the tested medicine can suppressed significantly the formation of the ex vivo thrombosis. The results showed Fu Fang Jiang Ya Pian might improve the rheology of blood stasis animals, which is good to preventing cardiovascular diseases.In mice' hyperlipemia model, Fu Fang Jiang Ya Pian was given po. for five days ,at the end of the fifth day, the total cholesterol, triglyceride were measured, the results showed that the difference between test and control groups was obvious, which indicated Fu Fang Jiang Ya Pian may decrease blood fat level of animals, and prevent indirectly the enhancement of hypertension.To sum up, Fu Fang Jiang Ya Pian can effectively decrease animal blood pressure and relief the symptom relevant to hypertension. Moreover, Its toxicity is little. Therefore, Fu Fang Jiang Ya Pian has a wide progress promising.