Expression And Significance Of P27 Cyclin D1 And Cyclin E In Bladder Transitional Cell Carcinomas

Objective To study the expression and it's significance of P27,cyclin D1 and cyclin E protein in transitional cell carcinoma of bladder(TCCB).Methods Expression of P27 cyclin D1 and cyclin E protein were investigated by immunohistochemistry technique (SP) with 65 cases of transitional cell carcinoma of the bladder, 10 atypical hyperplasia cases and 8 normal bladder cases.Results The postive immunostaining rate of P27 in normal bladder (100%) was significantly higher than in the atypical hyperplasia and significantly higher than in the TCCB (51%). The postive immunostaining rate of P27 was 75% of G1, 53.8% of G2, 26.3% of G3,72.9% of Ta-T1 and 25% of T2-T4.There was significant difference either among G1,G2,G3(P<0.05) or between Ta-T1 and T2-T4(P<0.01). cyclin E in the atypical hyperplasia(60%) was insignificantly higher than in the normal bladder tissue(25%). The expression rate of cyclin E was 80% of G1,42.3% of G2,31.6% of G3,62.2% of Ta-T1 and 35.7% of T2-T4. There was significant difference either among G1, G2, G3 (P<0.05) or between Ta-T1 and T2-T4 (P<0.01). The expression rate ofG1 was significantly higher than G2 and G3, The expression of cyclin D1 in the normal bladder tissue was negative. The expression rate of cyclin D1 in TCCB (38.5%) and in the atypical hyperplasia(40%)was significantly higher than that in the normal bladder tissue. The expression rate of cyclin D1 was 65% of G1, 34.6% of G2, 18.6% of G3,54.1% of Ta-T1 and 17.5% of T2-T4. There was significant difference either among G1, G2, G3 (P<0.01) or between Ta-T1 and T2-T4 (P<0.01). The expression rate of cyclin D1 of G1 was significantly higher than that of G2 and G3. P27, cyclin D1 and cyclin E were correlated with each other. Conclusions These findings suggest that the down or negative-expression of P27 and overexpression of cyclin D1 and cyclin E might accelerate the progression of the cell cycle and promote the carcinogenesis of the TCCB.The overexpression of cyclin D1 might be an relatively earlier event in the initition of the TCCB. With malignance of tumor increasing, the expression rate of them was decreasing. Analysis of P27,cyclin D1 and cyclin E expression in TCCB was useful for us to estimate the malignance and prognosis.