The Expression And Significance Of PLK1 And Cyclin B1 In Transitional Cell Carcinoma Of Bladder

Objective Bladder transitional cell carcinoma was the most common malignant tumor of the urinary tract, ranked fifth of cancer incidence rates and second mortality in urinary tract disease in the world. In recent years,there was a trend of increased incidence in the world, which had a multi-center, easy to relapse, and invasive growth characteristics .The comprehensive treatment based on surgery was used more currently, but the effect of treatment was not satisfactory,urgent needed for other effective treatment. In recent years the rapid development of molecular biology provided a theoretical basis to find new and effective method to treat bladder transitional cell carcinoma. Studies have shown that the incidence and development of bladder transitional cell carcinoma is the result of multi-gene and many factors, including cell cycle regulation,which plays an important role.Paul-like kinase 1 (polo like kinase 1, plk1) is a class of the expression of cyclin-dependent serine / threonine kinase and widely present in eukaryotic cells, plays an important role in mitosis and relates closely in cell proliferation, and links with some of the biological behavior of a variety of tumors and poor prognosis.This study investigated the expression of PLK1and Cyclin B1 in bladder transitional cell carcinoma and its clinical significance, may be provide a theoretical basis for the early diagnosis and treatment.Methods 41 cases of the resected specimens of bladder transitional cell carcinoma and 10 normal bladder tissues (more than 2cm away from the tumor adjacent normal mucosa) were collected. All specimens were fixed in formalin solution. All organizations were confirmed by pathology. Immunohistochemical methods(S-P) was employed to inspect the expression of PLK1 and Cyclin B1 in 41 transitional cell carcinoma of bladder and 10 bladder tissues, the contact between the expression of two protein and Clinic/Pathologic parameter, as well the correlation for the two protein were analyzed.Results The expression of PLK1 in bladder transitional cell carcinoma was brown-yellow or brown in the nucleus and other expression both in pulp and nuclear, The expression of cyclin B1 was brown yellow or brown in the cytoplasm. The positive expression rate of PLK1 and Cyclin B1 in transitional cell carcinoma of bladder was 51.2%(21/41)and 56.1%(23/41)respectively,but weak or no expression in normal bladder tissue . The difference was statistically significant (P <0.01). PLK1 expression had no correlation with age, sex, clinical stage,lymph node metastasis (P>0.05),but had correlation with pathological grades, and the expression differences of different pathological grade had statistically significant with the increased of clinical stage (P <0.05). The positive expression rates were 75.0% and 22.2%. Cyclin B1 expression had no correlation with age, sex, lymph node metastasis (P>0.05),but had correlation with clinical stage and pathological grades and increased with the increased of clinical stage, the positive rates were 40.0% and 71.4 %, the difference had statistically significant (P <0.05), while in different clinical stages, the positive expression rate of Cyclin B1 were rising with the increased of pathological grades, the difference was statistically significant (P <0.05). Furthermore, a correlation existed between PLK1 and Cyclin B1 in the transitional cell carcinoma of bladde(rr =0.317).Conclusion PLK1 and Cyclin B1 highly expressed and nearly correlated with pathological grades and clinical stage,suggested that they played an important role in the progression of the transitional cell carcinoma of bladder. Meanwhile, a regulating correlation could existed between PLK1 and Cyclin B1. Therefore, the two cytokines would provide a new theoretical basis for treatment research on the molecular level of bladder transitional cell carcinoma and could become a new target for cancer therapy.