The Expression Of Vegf,Flt-1 And Its Effect On MVD In Benign Prostatic Hyperplasia

Objective To study the MVD and the expression of VEGF,Flt-1 in benign prostatic hyperplasia (BPH), and analyze the effects on VEGF,Flt-1 and MVD in BPH.Methods Immunohistochemical staining (S-P) was used to examine MVD and the expression of VEGF,Flt-1 in 37 cases of BPH and 6 cases of normal prostate (NP). BPH cases was divided into 2 groups according to finasteride treatment or not. Immunohistochemical parameters were compared in both groups, and chisq test,student t test,Spearman rank correlation test and Wilcoxon test were used for data statistic analysis. Results 1. VEGF expression was negative in all 6 NP; in BPH, the expression of VEGF were negative in 8 cases (21.6%), (+)in 20 cases(54.1%)and (++) in 9 cases(24.3%), respectively. Statistic significance existed between the two groups (P<0.05). The expression of Flt-1 was (+) in all 6 NP, and (+) in 24 cases(64.9%), (++) in 13 cases(35.1%) in BPH, respectively. No significance existed(P>0.05). MVD parameter was 4.83±1.83 in NP and 8.00±3.28 in BPH, statistic significance existed between the two groups (P<0.05). 2. In BPH, the expression of VEGF correlated to both MVD and Flt-1(P<0.05), and Flt-1 did not correlate to MVD (P>0.05).There was no correlation between the size of prostate and MVD and the expression of both VEGF and Flt-1 in BPH(P>0.05). 3. In BPH, the expression of VEGF in the 19 patients treated with finasteride was (－) in 6 cases(31.6%), (+) in 13 cases(68.4%), respectively. In the 18 cases without finasteride, VEGF was (－) in 2 cases(11.1%), (+) in 7 (38.9%) and (++) in 9(50.0%), respectively. MVD was 6.89±2.54 in finasteride treated group and 9.17±3.63 in the nontreatment group. Statistic significance of both VEGF and MVD expression existed in the two groups (P<0.05). Flt-1 is (+) in 12 cases(63.2%), (++) in 7(36.8%) in treatment group, and (+) in 12(66.7%), (++) in 6(33.3%) in the nontreatment group, no significant difference existed in the two groups(P>0.05).Conclusions In the progress of benign prostatic hyperplasia, theexpression of VEGF and angiogenesis are significant developed. VEGF plays a necessary role in pathogenesis and development of BPH, but Flt-1 is a less important factor. VEGF is positive correlate to both Flt-1 and MVD. Finasteride can downregulate the synthesis and expression of VEGF, which inhibit the angiogenesis in BPH, but has less impact on Flt-1.