Study On The Immunology And Oocyte Donation Treatment Of Premature Ovarian Failure

Objective (l) 'lo investigate the levels of AOAb, ACA, 'l'G-Ab, EmAb, ASA inserum of patients with premature ovarian failure and the effects of them in the etiologyof POF. (2) 1b discuss the correlating factors of oocyte donation treatment that helpPOF patients to accquire pregnancy.Method (l) "I.he levels of AOAb, ACA, TG-Ab, EmAb, ASA in serum of 46 caseswith POF and l02 cases of normal controls were detected by indirect enzyme linkedimmunoabsorbeIlt assay, the result of each serum sample was expressed by the opticaldensity in 492l1m wavelength. The correlation analyses between the length ofamenorrhea time and the levels of 5 antibodies in serum of patients with POF wasperformed, so did the correlation between the levels of FSH, Lll and the levles of 5antibodies in serum of POF patients. (2) l5 POF patients were treated with hormonereplacement therapy and oocyte donation. The results of the treatment of differentoocyte source, tl1e donors and the recipients of IVF-ET surplus oocytes, the recipients of7x4I.different ages and embryo transfer window were analyzed.Result (l) 'I'he levels of AOAb(2.90I4.63), ACA(2.04 I2.70), 'I'G-Ab(l .42 I l .30),EmAb(2.77 14.38), ASA(l .62I2.87) in the serum of POF patients were higher thanthose in col1troIs significantly (0.42 I 0.6l, 0.46 l 0.62, 0.49 f 0.57, 0.55 f l .20, 0.52 f0.96) (P<0.00l ). 'l'here was significant correlation among AOAb, ACA, TG-Ab, EmAb,ASA in seruIn of POF patiellts. No correlations were fOund between the length ofamenorrhea time or the levels of FSH, LH with the levels of 5 an1ibodies in serum ofPOF patients. (2) In the oocyte donation treatment, the number of transIbrred embryos,cumulative embryo score and clinical pregnancy incidence in the donation group ofwhole oocyte colony were significantly higher than those of surplus oocytes of IVF-ETgroup (P<0.05--0.001 ), whi1e abortion incidence decreased significantly (P<0.00l), Thenumber of tral1sferred embryos and cumulative embryo score of donors weresignificantly hig[ler than those of recipients in IVF-ET surplus oocytes group (P<0.00l ),but the differences of clinical pergnancy incidence and abortion incidence between themwere not lbul1d (P>0.05). There was no difference in the number of transferred embryosand cumulative embryo score between the recipients <30 years and >30 years(P>0.05), but the clinical pregnancy incidence of younger group was significantlyhigher thal1 that of older group (P>0.05). Embryo transferring in l6--l8 day of IlRTperiod can accquire much more pregnancy (7/l9).Conclusion (l) The levels of AOAb, ACA, TG-Ab, EmAb, ASA in serum of POFpatients increase significantly .The change of immune system is one of the importantreasons in genesis and development of POF. (2) Hormone replacement therapy andoocyte donation is an effective method to he1p POF patients to acquire pregnancy.Oocyte source, period synchronism of donor and,recipient, hormone replacementtheraPy influence the success of the treatment.