| OBJECTIVES:The hemorrhagic shock (HS) is a common syndrome that presents the acute hepatic disease (AHD) and the acute renal failure (ARF), because of decreasing of the effective circulation blood volume and obstruction of perfusion microcirculation. 60 percent cases with trauma died from HS in the peacetime and wartime.The main treatment of HS requires initial infusion of a large volume of crystal and colloid solution immediately. The body temperature is usually affected by the biologic, physic and chemical factors in HS. So keeping the stable body temperature is the basic approach for resuscitation. Up to the present, the effects of solution type, volume and infusion sites have been reported, however the effect of solution temperature haven't been paid enough attention in resuscitation. To explore the effects of fluid temperature in resuscitation, microcirculation and hepatic or renal function were observed in rabbit HS model.METHODS30 new Zealand rabbits were randomly divided into four groups, including the control group(sham-operated) and three tested groups which were infused fluid and autologous blood in hypothermia(10.7 1.6 ), normothermia(20.6 1.3 ) and hyperthermia(39.5 1.3 ) respectively. The anus temperature were measured at different time points (5 min aftemacirsis, 10 min after catheterizing, 30 min inshock and 1h, 2h, 4h in resuscitation) , simultaneously the mean arterial pressure(MAP),bulbar conjunctiva microcirculation (the blood flow state, diameter and the number of microvessels) , hepatic (ALT, AST, TP, Alb) and renal functions (urine volume , urinomete, Urea, Cr, Urate) were observed except 5 min afternacirsis.RESULTS1. MAP: MAP was elevated after infusion of different temperature solution in the three tested groups, especially in the hyperthermic and normothermic groups at 2h and 4h resuscitation. MAP was elevated steadily in hyperthermic and normothermic groups, but it was not in the in hypothermic group.Anus temperature: Different changes were observed in the three tested groups during resuscitation. The anus temperature was higher in the hyperthermic group than the other two tested groups. (P=0.023,P=0.007)2. Bulbar conjunctiva (BC) microcirculation: The state of blood flow was improved in three tested groups with infusion. In 1h resuscitation , 7,1,1 of the slow granular flow and 1,6,7 of the granular flow was observed in hypothermic ,normothermic and hyperthermic groups respectively. And 1 of the linear granular was observed in hyperthermic group; In 2h resuscitation , 7,0,1 of the slow granular flow and 1,6,2 of the granular flow and 0,1,5 of the linear granular was observed in hypothermic ,normothermic and hyperthermic groups respectively, In 4h resuscitation, 2,1,1 of the granular flow and 6,6,7 of the linear granular flow was observed in hypothermic, normothermic and hyperthermic groups respectively.In 1h, 2h and 4h resuscitation, diameter of BC microvessels in thehyperthermic group was significantly larger than that in the other two tested groups. (P<0.05)In 1h, 2h resuscitation, no significant difference in the number of BC microvessels was got between hypothermic group and other two tested group. The number of BC microvessels was significant less than that of BC microvessels before and after shock in hypothermic group. In the hyperthermic and normothermic groups, the number of microvessels was gradually increased with time, especially in hyperthermic group.3. Hepatic functions: There are different changes in three tested groups after resuscitation. ALT was lower in the normothermic group and the hyperthermic group than that in the hypothermic group after 1hr postresuscitation, but it was higher in two groups than that in the hypothermic group. There was static significance of ALT level among the three groups after the 4h resuscitation.AST in the hyperthermic group was lower than that in other two groups after the Ih resuscitation. And AST in the normothermic group wa...
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