Genetic Polymorphisms Of Glutathione-s-transferase And Microsomal Epoxide Hydrolase With Susceptibility To Primary Hepatocellular Carcinoma In GuangXi

Objective To study phase â…¡ metabolic gene(GSTM1,GSTT1,mEH) polymorphisms and genetic susceptibility to primary hepatocellular carcinoma(PHCC) in GuangXi. Methods Polymorphisms of GSTM1,GSTT1 and mEH were analyzed in 105 patients with PHCC and 151 health controls by multiplex PCR ,PCR-RFLP and AMP-FLP. Results The frequency of the GSTM1 null genotype was found to be significantly higher among cases than that among controls(64.76% vs 50.99%,P<0.05),with the OR of 1.77 (95%CI =1.06-2.95) ,which suggested the individuals with GSTM1 null genotype has 0.77 times of increased risk of PHCC.The frequency of the GSTT1 null genotype showed no difference between the PHCC group and the control group(40.95% vs 33.11%,P>0.05).The frequencies of 3 genotypes for mEH in cases were 27.62%,21.90% and 50.48% respectively,while those of controls were 21.19%,34.44% and 44.37% respevtively.The differences were not statistically significant.To the length polymorphism of GSTM1 trinucleotide repeat sequence,nine alleles were detected and the most alleles were 8,10,43,53 in twogroups. The difference was not significant either(P>0.05).The individuals with both GSTM1 and GSTT1 deletion increased 1.22 times on the risk for PHCC .The OR was 2.22(95%CI=1.11-4.43).Conclusion No association with the risk of PHCC was observed for the GSTT1-null genotype or for the mEH polymorphism or for the GSTM1 trinucleotide repeat sequence polymorphism,whereas GSTM1-null genotype increased the risk of PHCC.GSTM1 and GSTT1 gene deletion were useful indicates for screening the high risk population of PHCC in GuangXi...