| IntroductionProstatitis is one of the common urological systemtic disease and usually occurs in youth and middle-age males. It has been estimated that up to half of all men suffer from symptoms of prostatitis at some time in all their lives. Benign prostatic hyperplasia(BPH) is one of the most common medical problems in the elderly men. As the prostate becomes enlarged, with squeezes the urethra and narrows the tube and can cause morefrequent urination, difficult urinating and even blocking of urination. BPH morbidity is approximately 30%-50% at fifty and 75% at sixty-seventy in men. There is an increase trend of the prostatitis and BPH occurrences in recent years. Given the complexity of the etiology and pathology of prostatitis and BPH, up to now it has not been completely elucidated yet. And there are no medications and therapies available to treat the conditions effectively. So it is of great importance to search for some effective therapy drugs.Bamboo leaves contain a lot of flavonoid, phenic acid, anthraquinone, polysacchande, coumarinic lactone, special amino acid, aromatic components and microelements, such as manganese, zinc, selenium, etc. Bamboo leaves flavonoid extract(BLFE) is obtained from phyllostachys and 50% is flavonoid, main component of which is flavone C-glucoside, primarily including orientin, homoorientin, vitexin and isoviextin. It has been confirmed by pharmacological study that BLFE has effects of anti-free radical, anti-lipid peroxidation, protectingDNA from oxidizing damage, and decreasing triglyceride level and increasing high density lipoprotein cholesterol(HDL-c) level in serum. In ancient and modern Chinese traditional medicine documents, it is recorded that bamboo leave could be used to treat prostatitis and BPH. However, to our knowledge, there is no available report on pharmacological effects of BLFE on prostatitis and BPH.Objectives: To evaluate the efficacy of BLFE for the treatment of prostatitis and BPH. The anti-bacteria experiment in vitro, multiple inflammatory model in rats and mice were used to assess the anti-bacteria and ant-inflammation activity of BLFE. Testosterone propionate-induced BPH model in mice was also used to observe the effects of BLFE on exogenous hormone induced BPH. And using growth test of prostate, gonad and accessory sex organs in juvenile mice, the effects of BLFE on normal gonad growth as well as the direct effects on prostate were observed. So this study provided a basis to further extract, purifyand utilize the effective components in bamboo leaves, and that is to evaluate BLFE whether or not to exert anti-prostatitis and anti-BPH.Methods:1. Minimum inhibition concentration test (MIC test)(1)Streptococcus Pyogenes: BLFE was added to the sterile M-H broth with 10% inactivated calf blood serum to a final concentration of 25.0mg/ml. Then filtration sterilization was carried out. After that the culture was diluted with culture media to a series with grades of half concentration, added with 0.05ml of young Streptococcus pyogenes solution(with total bacteria amount of 104c.f.u) and incubated at 35 C for 20-24 hr to obtain the value of MC.(2)Enterococcusfaecalis, Staphylococcus epidermidis, Proteus vulgahs, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli.: Carried out with series drug dilution of half concentration. M-H agar was liquefied after autoclaving andcooled to about 55 C. The solutions of the above 6 bacteria were spotted to each dish of the M-H agar with series concentrations of drugs respectively. After dry out, the cultures were incubated at 35 C for 16-20 hr to obtain the value of MIC.2. Croton oil induced ear swelling in miceBLFE at 400 mg/kg, 200mg/kg and 100mg/kg was intragastrically administrated once a day for 7 consecutive days. Indomethacin was intragastrically administrated as positive control at 10mg/kg once before test and negative control was equal volume of water. One hour after final administration, 2% of Croton oil was smeared on the righ... |
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