| The incidence of brain injury was increased yearly with the development of the economy and transportation. Its mortality rate and mutilation rate were also enhanced. The statistics showed, in Chinese cities, patients dying from craniocerebral injury had amounted to 60% of all dead from the trauma. Recently, brain injury occurred more and more frequently, and has become the first one of most factors that lead to the death in the less than 45-aged individuals. Up to now, surgical management is most pivotal to craniocerebral injury, and, however medication still is very important. Therefore, any kind of effective remedy is very necessary.Free radical abnormal reaction occurs in the bodies of patients with craniocerebral injury, and is one of most factors inducing secondary damages. In bodies of patients with craniocerebral injury, the markedly increased content of free radicals resulted in serious disorders, the dynamic balance between oxidation and antioxidation produced grave imbalance, and the oxidative stress caused serious pathological aggravation. Lipoperoxidative reaction in plasma and tissues, especially in erythrocytic membranes containing a large number of polyunsaturated fatty acids,can lead to generation of a large number of free radicals. LPO is an important poisonous residual product, and can strongly attack DNA, proteins, enzymes, biological membranes, and others in the human body. Superoxide dismutase(SOD) is the most important antioxidase in the human body, and play important roles in scavenging oxygen free radicals and in preventing physiological and pathological aggravation of a series of free radical chain reactions, thereby protecting biological membranes of cells against oxidative and lipoperoxidative damages. Marked decrease of the activities of SOD in the human body can cause metabolic disorders and pathological aggravation of a series of free radical chain reactions, thus inducing a variety of diseases related to the abnormal reactions of free radicals. Some authors reported that the content of LPO in the bodies of patients with craniocerebral injury markedly increased, while SOD significantly decreased.However, recently, there are reports on the therapeutic effect of naloxons to patients or animals with craniocerebral injury, while there are few reports on changes of free radicals in the bodies of patients with craniocerebral injury and with injection of naloxone. To investigate the abnormal reactions of SOD and LPO and the influence of small or high doses of naloxone to them in the bodies of patients with craniocerebral injury, 60 healthy rabbits were enrolled in a random control study and divided into control group, high dose group and small dose group, respectively with 10 pairs of rabbits. Improved Feeney free dropping objective mode is used to produce severe craniocerebral injury of all rabbits. After formation of models, NS, naloxone injection of high dosage or small dose were administered systemically intraperitoneally to three groups of rabbits respectively in 2 hours, and fasting venous blood samples were collected from all the subjects in 6 hour, 1day, 3day, 7day, 11 day and 15day. Activities of SOD in erythrocytes and concentration of LPO in erythrocytes were determined with spectrophotometric assays. At the same time, the differences among average values of SOD and LPO between three groups and within three groups were compared. Additionally, the reliability coefficient(alpha) used for the above parameters to reflect the oxidative damages in the bodies of rabbits with severe craniocerebral injury with or without naloxone injection wasestimated.Materials and methods1, Subject: 77 healthy rabbits were divided randomly into normal group, control group, high dose group and small dose group respectively with 17, 20, 20, 20. There was no significant difference among the average values of body weight in four groups by F test (P=0.6009) .2, Model: Except normal group, in three groups, a bone window was opened in left parietal bone (diameter: 8.0mm). An... |
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