The Expression Of Transcription Factor GATA-1, GATA-2 And GATA-3 Genes In Bone Marrow Stromal Cells From Patients With Leukemia Or Aplastic Anemia

Objective: To investigate the expression of transcription factor GATA-1, GATA-2 and GATA-3 genes in the bone marrow stromal cells (BMSCs) from normal people and patients with hematologic diseases. This study is expected to provide some basic data of molecular mechanism by which bone marrow microenvironment influences hematopoiesis.Methods: The expression of GATA genes were analyzed by using RT-PCR-ELISA in the BMSCs from 34 normal cases and 59 cases with hematologic diseases(45 cases with leukemia, 9 cases with aplastic anemia ( AA ) , 3 cases with myelodysplastic syndrome (MDS) and 2 cases with malignant histiocytosis) , also in the bone marrow hematopoietic cells from 25 normal cases and 45 cases with hematologic diseases. The semi-quantitative expression levels of GATA genes in the BMSCs and the bone marrow hematopoietic cells from patients with leukemia or AA were compared with normal controls, respectively. And the expression patterns of GATA genes were analyzed.Results: It was successful to establish the isolation, amplification, cultivation andidentification of the BMSCs in vitro. The expression of GATA-1, GATA-2 and GATA-3 genes could be detected in the BMSCs and the bone marrow hematopoietic cells from both normal controls and the cases with hematologic diseases. The expression rate of GATA-1 in the BMSCs from acute lymphocytic leukemia (ALL) was similar to the normal controls, whereas the expression rates in BMSCs from AA, acute myelocytic leukemia (AML) and chronic myelocytic leukemia (CML) were significant lower than the normal controls(P<0.05). The ranks of expression levels of GATA-1 and GATA-3 genes in the BMSCs were "ALL>AML>normal>CML>AA" and "AML>ALL>AA>normal>CML", respectively. It was no difference in the expression levels of GATA-2 gene within the BMSCs from normal controls and AA and leukemia. However, the ranks of expression levels of GATA-1, GATA-2 and GATA-3 genes in bone marrow hematopoietic cells were "AML>AA>normal>ALL>CML", "AML>CML>ALL>normal>AA" and "AA>ALL>normal>AML>CML", respectively. The dominant expression of GATA-2 gene was found in the normal BMSCs, whereas the dominant expression of GATA-3 gene was detected in the BMSCs from AML or AA.Conclusions: The expression of GATA-1, GATA-2 and GATA-3 genes could be identified in the BMSCs from normal controls and patients with leukemia or AA, but their expression levels and patterns may change in different conditions. It may influence the regulation of hematopoiesis in the BMSCs and play the role in pathogenesis and development of leukemia or AA.