Expressions And Significances Of VEGF And BFGF In Bone Marrow Of Aplastic Anemia Patients Prior And Post Treatment

Objective:To investigate the expression of two important angiogenesis factors-vascular endothelial growth factor(VEGF) and basic fibroblastic growth factor(bFGF) in marrow microenvironment-marrow stroma of aplastic anemia(AA) patients prior treatment and post remission,and approach the contribution of marrow stroma on the pathogenesy of AA.Materials and Methods:32 biopsy specimen of bone marrow from AA patients prior treatment and post remission were involved in this study,who were all patients of our hospital from Jan,2002 to Dec,2007,males 18 cases and females 14 cases,aged from 11 to 49 years old,with average age of 36 years.10 SAA and 22 NSAA cases were involved with exact diagnosis of Diagnosis And Therapeutic Effect Denominator of Hematonosis by Zhang Zhi-nan.10 control group specimen of costal bone marrow from chest surgery were studied in this experiment,males 6 cases and females 4cases,aged from 19 to 42 years old,with average age of 33 years.The expression of VEGF and bFGF was checked with immunohistochemistry technique and the stactistics analysis was done to evaluate whether there was significant difference between their expression levels.Results:(1) The expression levels of VEGF and bFGF in bone marrow of SAA patients prior treatment were obviously lower than control group specimen (t=21.03,p＜0.001;t=22.9,p＜0.001)and those post remission were significantly higher than prior treatment(t=22.86,p＜0.001;t=21.88,p＜0.001),which showed no more manifest difference than control group specimen(t=0.75,p＞0.05;t=0.26,p＞0.05).(2) The expression levels of VEGF and bFGF in bone marrow of NSAA patients prior treatment were obviously lower than normal tissue(t=18.71,p＜0.001;t=22.3, p＜0.001)and those post remission were significantly higher than prior treatment(t=18.77, p＜0.001;t=21.84,p＜0.001),which showed no more manifest difference than control group(t=0.05,p＞0.05;t=0.18,p＞0.05).(3) There were no significant differences in the expressions of both VEGF and bFGF between SAA and NSAA patients prior treatment(t=1.25,p＞0.05;t=1.58,p＞0.05).After effective therapy their expressions all achieved the level of control group and likewise showed no significant differences between SAA and NSAA(t=0.93,p＞0.05;t=0.41,p＞0.05).(4) A positive correlation existed between these two expressions(AA prior treatment r=0.891,p＜0.01;AA post remission r=0.917,p＜0.01;control group r=0.89,p＜0.01 ).Conclusion:1.Prior treatment the expressions of VEGF and bFGF in bone marrow of both SAA and NSAA patients notably decreased,which explained that the marrow stroma was damaged with the invasion of AA.But post remission their expressions increased so greatly that it achieved the level of normal control group, which stated the recovery of marrow stroma followed the healing of haematogenesis.2.The damage of marrow stroma was one of pathogenesis in AA and it's improvement may offer an original and effective method for the therapy of AA.