The Correlation Study On Serum Ferritin, Folic Acid And Vitamin B₁₂ In Cord Blood And Pregnant Woman Peripheral Blood

ObjectiveWe research the effect of maternal anemia on fetus, infant and pregnancy outcome. We research the relation between maternal anemia and birth weight. We assess the etiologic factors responsible for anemia in pregnancy. Research the content of serum ferritin, folic acid and vitamin 812 in maternal blood, the relative between maternal blood serum ferritin, folic acid and vitamin 812 and those in umbilical cord blood. Iron, folate and vitamin 812 deficiency and birth weight. MethodBetween March and October Sixty women who were pregnant for the first time were recruited from the Air Force Hospital in Wuhan, at 37-42 weeks' pregnant. None of the 60 women enrolled in this study had clinical signs or symptoms suggestive medical or surgical conditions other than anemia. They had not take iron, folic acid and vitamin 812 supplements, were no smoking. The obstetric data included maternal age gestational at deliver (estimated from the last menstrual period and ultrasound scan), infant sex, birth weight. At delivery, maternal and cord blood samples were collect. Serum fractions were collect by centrifugation (1500 x g at room temperature for 5 minute) and stored at -20 C. Full blood counts were obtained by using an automated cell counter. Serum ferritin, vitamin 8-12, and serum folate, concentrations were analyzed by radioimmunoassay techniques. All data were presented as means ?standard errors. Microsoft Excel 97 was used to data processing. Student's test for independent samples and the chi-square test were used as statistical methods, whereappropriate. Pearson's correlation was used to evaluated the correlations among maternal and cord blood iron, folic acid and vitamin 612. Data analyses were done with SAS statistical software (SAS Institute, Gary, NC). A P-value of <0.05 was considered statistical significant. ResultMean hemoglobin concentration in maternal blood was 108+ 12.3g/L, 28 women's hemoglobin concentration < 1 lOg/L, in cord blood was 171 + 13.7 g/.Mean hemoglobin concentration in cord blood that the infants' mothers were anemia and normal was 167. 4 + 14.5 g/L, 173 + 11.4 g/L respectively. The difference was evaluated by student's t-test and was found to be no significant. Mean birth weight of the infants was several anemia 3048.33 g, which was lower than those of the infants whose mothers were normal. Among the 28 women who were anemia, 17 women were iron deficiency anemia, 8 women were folic acid deficiency and others were vitamin B12 deficiency. Mean serum ferritin levels in maternal and cord blood were 1.82 +5.2 U g/L and 117.8 +20.4 U g/L respectively. The difference was evaluated by student's t-test and was found to be significant. The cord blood serum ferritin levels of infants whose mothers' serum ferritin levels was lower had no difference with those whose mother' serum ferritin levels were normal. The serum ferritin levels of cord blood were not correlation those of maternal blood. Mean folic acid levels in cord blood and maternal blood were 8.20+2.89nmol/L and 11.71 +3.78nmol/L, they had correlation(r=0.75, p-value < 0.01). Mean vitamin B12 levels in cord blood and maternal blood were 121.7 + 20.8pmol/L and 429.5 +26.6 pmol/L respectively. Conclusion1 .Anemia was sill a several complication in pregnancy. Iron deficient anemia was still the commonest anemia in pregnancy.2.1nfants whose mothers were anemia were not anemia, maternal anemia didnot affect on infant hemoglobin concentration.3. Mothers of lower birth weight infants had lower hemoglobin concentration, were several anemia.4. Serum ferritin levels of cord blood are no correlation with those of maternal blood. Folic acid and vitamin 812 levels of cord blood are correlation with those of maternal blood.5. Iron, folic acid and vitamin 612 deficiency of pregnant women don't affect neonatal serum ferritin, folic acid and vitamin 812 levels.6. Iron, folic acid and vitamin 812 deficiency of pregnant women don't affect neonatal birth weight.