The Research On Mechanism Of Angiogenesis In The Primary Liver Carcinoma

The Research on Mechanism of Angiogenesis In The Primary Liver CarcinomaAngiopoietin-2(Ang-2) is recently recognized as a secreted glycoproteinwhich is closed to angiogenesis in carcinogenesis and effects the neoplasm development and metastasis. Primary liver carcinoma that has been threatening human health and life appears hypervascular characters. To reach the aim of revealing the correlations among angiopoietin-2, malignant biological behavior and angiogenesis in primary liver carcinoma, angiopoeitin-2 expression levels in 30 specimens of liver carcinoma and tissue surrounding tumor were assayed with Quantitative Reverse Transcription Polymerase Chain Reaction (Q-RT-PCR) and immunohistochemical method. Meanwhile CD-34 protein expression was determined with immunohistological assay and then to be used to evaluate micro vessel density (MVD). The correlation between angiopoeitin-2 and MVD was analyzed.The result showed that:1. The Angiopoietin-2 mRNA and protein expression levels were significantly higher in liver cancer than that in tissue surrounding tumor.2. The significant differences of angiopoeitin-2 expression in different invasion groups were found. These levels were significantly higher in invasion subgroup than that in non-invasion subgroup.3. The angiopoeitin-2 expression levels were significantly relative with tumor cell differentiation and apparently higher in poor differentiation subgroup.4. The CD-34 protein expression was significantly higher in liver carcinoma than it in tissue surrounding tumor.5. The was significance of CD-34 expression between invasion andnon-invasion subgroups of liver carcinoma. This level was higher in invasion subgroup.6. The significant difference of angiopoietin-2 protein expression was found in different MVD subgroups. The expression level was significantly higher in the high MVD subgroup. There was a significant correlation between angiopoietin-2 protein expression and MVD.7. No any relation was found between angiopoietin-2 and tumor location, histology, gross type or AFP level.The investigator concludes that:1. Angiopoeitin-2 plays an important role in the occurrence and development of liver carcinoma. Angiopoietin-2 is apparently relative to the invasion, histology and differentiation of liver carcinoma.2. MVD has a significant correlation with the liver carcinoma invasion. Angiogenesis is a very important factor in the progression and metastasis of liver carcinoma.3. The significant correlations between liver carcinoma invasion and angiopoietin-2 and/or MVD suggest that these two cyto-molecular agents may be relative to the biological behavior of liver neoplasm. It is necessary to be further studied if angiopoeitin-2 and MVD could serve as a marker to evaluate the prognosis of the patient with liver carcinoma.