The Expression Of Survivin, NF-κB And Nm-23h1 Protein In Hepatocellular Carcinoma

Objective: To observe the expression of apoptosis inhibitor protein Survivin, nuclear transcription factor-K B( NF-K. B) and nm-23hl protein in the hepatocellular carcinoma(HCC) and approach their significance and try to disclose the the original mechanisms of HCC and to approach the relationship between the three protein with clinicopatho-logic feature of patients with HCC. Methods: the expression of Survivin protein, NF-K B protein and nm23-hl protein in 41 cases of HCC patients were examined immunohistochemically, taking 41 cases of corresponding non-cancerous adjacent tissues, 11 cases of liver cirrhosis tissues and 11 cases of normal liver tissues from resected liver benign hemangioma specimen as control. Results: 1. Survivin was expressed in 48.8% cases of HCC tissues, in contrast, no expression was found in controlled tissues (p<0.01); Survivin was expressed in 88.9%(8/9) cases with portal venous invasion in HCC tissues compared with 37. 5%(12/32) cases with no portal vein invasion (p<0. 05); Survivin was expressed in 41%(12/30) cases of single tumor HCC tissues compared with 72.7% cases of multiple tumor HCC tissues (p<0.05). Expression of Survivin protein correlated with portal venous invasion and number of tumor in HCC patients. 2. NF-K B protein was detected in 68.3%(28/41) cases of HCC tissues compared with 12. 2%(5/41) in non-cancerous adjacent tissues, 1/10 cases in liver cirrosis, 0/11 cases in normal liver tissues (p<0.01). NF-KB expression has no correlation with clinicalpathological feature of HCC patients but correlates closely with Survivin expression (p<0.05). 3. Four cases of 41 HCC tissues were found nm-23hl protein positive staining, compared with 27 cases negative staining; negative expression of nm-23hl protein correlates closely with portal vein invasion and serosal infiltration (p<0.05), and its negativeexpression correlates well with Survivin expression (p<0.01). Conclusion: 1 High expression of Survivin protein and NF-KB protein in HCC tissues suggests that it probably play an important role in the origin and development of HCC, and it may be as targets for gene therapy. 2 Positive expression of Survivin protein and negative expression of nm-23hl protein correlates with malignant biological feature of HCC, and it probably suggests poor prognosis. 3 Correlation of Survivin, NF-KB and nm-23hl suggests they co-affect in the origin and development of HCC.