The Expression And Their Significance Of Survivin PTEN And NF-κB Protein In Hepatocellular Carcinoma

Objective: To explore the mechanisms and significances of Survivin, PTEN and NF-κB genes in hepatocellular carcinoma(HCC) and surrounding nonmalignant hepatic tissues by investigating their protein expression, combination with characteristics of clinic and pathology.Methods: Immunohistochemical technique was used to detect the protein expression ratio and intensity of Survivin, PTEN and NF-κBp65 in 30 specimens of HCC, surrounding nonmalignant hepatic tissues and 10 specimens of normal liver tissues.Results: The expression of Survivin protein in human HCC, surrounding nonmalignant hepatic tissues and normal liver tissues were 60.00% (18/30), 23.33%(7/30) and none(0/10); The expression of Survivin protein in human HCC were both significantly higher than surrounding nonmalignant hepatic tissues and normal liver tissues(all P<0.0125); But it was not statistically significant difference between surrounding nonmalignant hepatic tissues and normal liver tissues (P >0.05). The expression of PTEN protein in human HCC, surrounding nonmalignant hepatic tissues and normal liver tissues were 56.67% (17/30), 93.33%(28/30) and 100%(10/10); The expression of PTEN protein in human HCC were both markedly lower than surrounding nonmalignant hepatic tissues and normal liver tissues(all P<0.0125); But it was not statistically significant difference between surrounding nonmalignant hepatic tissues and normal liver tissues (P >0.05). The expression of NF-κBp65 protein in human HCC, surrounding nonmalignant hepatic tissues and normal liver tissues were 66.67% (20/30), 13.33%(4/30) and 10.00%(1/10); The expression of NF-κBP65 protein in human HCC were both markedly higher than surrounding nonmalignant hepatic tissues and normal liver tissues(all P<0.0125); But it was not statistically significant difference between surrounding nonmalignant hepatic tissues and normal liver tissues (P >0.05). The expression of Survivin and PTEN protein in HCC were significantly correlated with portal vein invasion(P<0.05), but no correlated with age, HBsAg, AFP, the tumor size, histologic differentiation and clinical stage (P>0.05). The expression of NF-κBp65 protein in HCC were no correlated with all of the clinical parameters (P>0.05). Survivin protein expression in HCC was inversely associated with PTEN protein expression(r=-0.655, P<0.05) and there was positive correlation between Survivin protein and NF-κBp65 protein expression in HCC(r=0.361, P<0.05).Conclusions: The abnormal expression of Survivin, PTEN and NF-κBp65 protein in HCC sugguest that they probably play important roles in the origin and development of HCC and their possible values as molecule targets in diagnosis and therapy may be researched further. Survivin protein expression in HCC was inversely associated with PTEN protein expression, which probably participate in the origin and development of HCC via survivin activation, up-regulation and PTEN inactivation, down-regulation. Survivin protein expression in HCC was positive associated with NF-κBp65 protein expression, which suggest that they may have a synergistic effect in the origin and development of HCC.