| Gardenia Jasminoides Ellis, Rubia cordifolia plant's dry mellow fruit, can reduce blood pressure, protect liver, be secretory bile, stanch and so on in Traditional Chinese Medicine theory and can relieve hypertension, diabetes, hepatitis in clinic. Geniposide, one of bioactive components, has been used to control the quality of Gardenia Jasminoides Ellis in Chinese Pharmacopoeia (2000 edtion, the first part). But determination of the content of geniposide in many preparations including Gardenia Jasminoides Ellis such as Jiaweixiaoyao pill and Qingkailing injection may give one guide line for their quality control. The paper studied the method with RP-HPLC to determine the geniposide in Gardenia Jasminoides Ellis fruit, peel, seed, Jiaweixiaoyao pill and Qingkailing injection, synchronously. It provides one quick, simple method to control quality of Gardenia Jasminoides Ellis and the preparations containing Gardenia Jasminoides Ellis.Objective:To establish a method for the assay of geniposide in Gardenia jasminoides Ellis and the preparations containing Gardenia jasminoides Ellis in order to provide one guide line for their quality control.Methods: ①Separate and extract geniposide from Gardenia jasminoides Ellis fruit, peel, seed, Jiaweixiaoyao pill and Qingkailing injection by supersonic wave-extraction.②Choose appropriate column, different formulation and proportion of mobile phase and adjust optimal flow rate and wavelength in order to separate geniposide well.③Preparation of standard curve: prepare a series of the reference solution, determine peak area,then the regression equation was obtained with the content of geniposide as abscissa, the relevant peak area as ordinate. ④Establishment of the lowest detection limitation: Dilute the reference solution until the value of S/N is more than 3. The relevant concentration is the lowest detection limitation. ⑤In the experiment of stability, transfer the sample solution from Gardenia jasminoides Ellis and the preparations containing Gardenia jasminoides Ellis to determine the relevant peak areas at 1, 2, 3, 4, 5, 6, 7 and 8 hour, respectively. ⑥In the experiment of precision, take the same test solution, inject it into the apparatus for six times, determine the peak areas of geniposide and calculate the concentrations of geniposide according to the same standard curve with the same apparatus in the same day, record the chromatograms, thencalculate the precision. ⑦In the experiment of reproducibility, prepare one concentration sample solutions of Gardenia jasminoides Ellis and the preparations containing Gardenia jasminoides Ellis, repeat each concentration solution five times in the same way, respectively. ⑧In the experiment of recovery, transfer 5 shares of Gardenia Jasminoides Ellis and the preparations containing Gardenia jasminoides Ellis , respectively, add same volume of reference solution of geniposide. Inject them into the apparatus and determine the content of every sample. ⑨The samples of Gardenia Jasminoides Ellis fruit, peel, seed, Jiaweixiaoyao pill and Qingkailing injection were analyzed under above-mentioned conditions.Results: ①The method of supersonic wave-extraction was simple, quick and stable. ②The HPLC system was carried out on Kromasil C18 (4.6mm×250mm, 5μm) with acetonitrile-water (10:90) as a mobile phase, and the geniposide was detected at wavelength of 238 nm. Under the above circumstance, the peaks of these samples were separated well with the resolution of more than 1.5. Theoretical plates was more than 6000 and the tailing factor for the peak of geniposide was between 0.98 ~ 1.04. The result showed that the negative samples did not affect the determination of geniposide. ③The regression equation was: Y=4.9662X-0.0515, r=0.9998 (n=5), the linear range of geniposide was from 0.596μg to 2.980μg. ④The lowestdetection limitation was 11.2ng. ⑤The samples were stable for 8 hours, and the relevant RSD values were 1.5%, 0.75% and 1.8%, respectively.⑥ The precision of sample was g...
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