Study On The Correlation Between Different Processing Technology And Anti-inflammatory Effect Of Gardenia Jasminoides Ellis

Objective: In this study,Gardenia was taken as the research object,and thermal analysis technology was used to analyze and study the pyrolysis process of Gardenia and its different effective components,and optimize the processing technology of different processed products(Fried Gardenia,Fructus Gardeniae Praeparatus,Gardenia Carbon);The network pharmacology method and jaundice hepatitis animal model experiment were used to study the correlation between different processing technology and anti-inflammatory effect of Gardenia jasminoides Ellis.Methods: 1.TG-DTG technology was used to study the pyrolysis and combustion characteristics of Gardenia Total Iridoid terpenoids extract,Gardenia total flavonoids extract,Gardenia total organic acids extract,gardenoside control,chlorogenic acid control,ursolic acid control,gardenia original medicine,Gardenia extract,and analyze different processed Gardenia products(Fried Gardenia,Fructus Gardeniae Praeparatus,Gardenia Carbon)Optimum processing temperature range.2.On the basis of thermal analysis experiment,response surface design method was used to optimize the processing temperature and time of different processed Gardenia products(Fried Gardenia,Fructus Gardeniae Praeparatus,Gardenia Carbon),and the best processing technology was obtained.3.Using the method of network pharmacology analysis,through a variety of related network databases,the effective components of Gardenia were collected,the active compounds and target proteins related to anti-inflammatory were screened,the genes related to anti-inflammatory were collected,the Wayne map,drug / compound target disease network map,protein interaction network(PPI)map,and the hair of Gardenia were constructed the potential core target pathway of antiinflammatory effect was analyzed.4.The jaundice hepatitis induced by ANIT was used as the inflammatory model.The blank group,model group,Fried Gardenia group,Fructus Gardeniae Praeparatus group and gardenia group were respectively set up for pharmacodynamic test.The expression levels of serum AST,ALT,total bilirubin,IL-6 and other cytokines were detected in each group.The liver of each group was examined for histopathology.The gastrointestinal microecology changes of each group were compared by 16 SrRNA sequencing To analyze the correlation between the different flora and the anti-inflammatory effect of Gardenia jasminoides Ellis,and to further explore the anti-inflammatory mechanism of Gardenia jasminoides Ellis by combining the prediction results of network pharmacology and related pharmacodynamics experiments.Results: 1.According to the analysis of the pyrolysis characteristic curve of the effective components of gardenia,the optimum processing temperature range of Fried Gardenia is 165.9℃~200℃,the optimum temperature range of Fructus Gardeniae Praeparatus is 216.2℃~290℃,and the optimum processing temperature range of Gardenia Carbon is 290.3~387.0 ℃.2.Through response surface experiment optimization,the best processing technology of Fried Gardenia is as follows: frying temperature 179℃,frying time 5.70 min;the best processing technology of Fructus Gardeniae Praeparatus is as follows: frying temperature 240℃,frying time 3.7 min;the best processing technology of Gardenia Carbon is as follows: frying temperature 329.94℃,frying time 5.91 min.3.Network pharmacology predicted 56 compounds related to anti-inflammatory activities,215 genes related to anti-inflammatory functions,insulin(INS)and protease B1(AKT1)as the PPI network center of Gardenia and antiinflammatory functions,and the analysis of the enrichment results of KEGG metabolic pathway showed that 20 high-frequency common KEGG pathways related to antiinflammatory effects of gardenia,including PI3K-Akt signal pathway and MAPK The high frequency of signaling pathway,FOXO signaling pathway and NF-kappaB signaling pathway indicates that Gardenia jasminoides Ellis plays an anti-inflammatory role closely related to these pathways.4.Compared with the blank group,the levels of AST,ALT,total bilirubin and IL-6 in the model group increased significantly(P < 0.01);Compared with the model group,the levels of AST in the serum of mice in the three groups were significantly decreased(P < 0.05);The content of ALT in the serum of the mice in the Gardenia group and the stir Fried Gardenia group decreased significantly(P < 0.05);Compared with the model group,the expression level of IL-6 in the serum of the mice in the Gardenia group decreased significantly(P < 0.05),but there was no significant trend in the Gardenia group and the Gardenia group;Compared with the model group,the total bilirubin content in the serum of the mice in the Gardenia group and the Fried Gardenia group decreased significantly(P<0.05),but there was no statistical difference between the two groups.The pathological sections of the liver of mice in each group showed that the pathological changes of the Raw Gardenia group and the Fried Gardenia group were mild,and the liver of the model group was necrotic and the structure was destroyed.The liver necrosis of Raw Gardenia group and Fried Gardenia group was mild,and there was inflammatory cell infiltration.The liver necrosis of the Fructus Gardeniae Praeparatus group was serious.Compared with the blank group,the abundance of Lactobacillaceae in the model group decreased(P < 0.05);The abundance of bacteroideae(P<0.05),Enterobacteriaceae,Helicobacteriaceae,Enterococceae increased,but there was no statistical difference;Compared with the model group,the abundance of Lactobacillaceae in the Gardenia group,the Fried Gardenia group and the Fructus Gardeniae Praeparatusg roup increased significantly.The abundance of Bacteroidaeceae in Gardenia group and Fried Gardenia group decreased without statistical difference,but there was no significant change in Fructus Gardeniae Praeparatusg;The abundance of Enterobacteriaceae,Enterococcaceae and Helicobacteraceae in Gardenia group and Fried Gardenia group did not decrease,but the abundance of Fructus Gardeniae Praeparatusg group decreased significantly,approaching to the blank group,no decrease Statistical difference.The results of intestinal microecology showed that lactobacilli may play an important role in the process of anti-inflammatory of Gardenia jasminoides Ellis.Conclusions: In this study,the thermal analysis experiment was used to study the effective components of Gardenia jasminoides Ellis and the pyrolysis characteristics of the original medicinal materials.The optimal processing temperature range of different processed products(Fried Gardenia,Fructus Gardeniae Praeparatusg and Gardenia Carbon)was obtained.On this basis,the process optimization was carried out and the process parameters of different processed products were quantified.Compared with the traditional processing methods,the quality of decoction pieces could be controlled.The anti-inflammatory effect of the optimized processing technology was studied by two systematic biological research methods of network pharmacology and intestinal microecology experiment,and the internal relationship between the processing technology and the efficacy mechanism was found,which is helpful to further reveal the processing mechanism of Gardenia.Through the combination of thermal analysis experiment and response surface experiment,the processing technology of traditional Chinese medicine is studied,and the processing mechanism is studied by systematic biological method.The method is reasonable and feasible,which can provide reference for improving the traditional processing technology in the future and reasonably quantifying the fire parameters in the process of processing.