| Purpose:To study the influence of Tetramethylpyrazine (TMP) and Ciprofloxacin on the proliferative vitreoretinopathy(PVR) and their mechanism, then to estimate the possibility of clinical application for provention and cure PVR.Method: 1. To culture human conjunctiva fibroblast cells by trypsin digestion method and describe their growth features in vitro.2. the function of TMP and Ciprofloxacin on the cell proliferative activity was evaluated by MTT method. Cell morphology was observed under the inverted light microscopy.3. 18 New Zealand white rabbits were put into three groups randomly. The left eyes of each rabbit were control group.The right eyes each of the 18 rabbits received an intravitreal injection of Tetramethylpyrazine or Ciprofloxacin. The eyes were evaluated clinicallyby means of slit-lamp examination and direct ophthalmoscopy before and after the intravitreal injection. The rabbits were killed after 24hs for light microscopic histopathologic analysis.4. 50 rabbits were divided into 5 groups randomly.repeat to inject the same deal dosage medicine into the rabbit`s vitreous body After surgery 3 days. The fundus was examined immediately and was checked for hemorrhages or surgically induced detachments. Slit lamp biomicroscopy and direct ophthalmoscopy were repeated and recorded at 1,3,7,14,21 and 28 days after the blood injection. Results: 1. The index number growth period of the human`s conjunctiva fibroblast cells is from after inoculating 24 hs to the 7th day, and the 7th day is platform period.2. The cultured conjunctiva fibroblast cells proliferation was significantly inhibited by TMP and Ciprofloxacin. And the effect were dose dependent. The IC50 values of TMP and Ciprofloxacin on conjunctiva fibroblast cells on 48hs were 1.36mg/ml and 12.77ug/ml respectively.3. After an intravitreal injection of TMP or Ciprofloxacin, there were bubble-like degeneration in these rabbits retinas in the 12mg Ciprofloxacin group. No evidence of toxic effects based on the clinical examinations and light microscopic examination in the others groups wasobserved.4. After 14 days, the vitreous bodies were changed from gray-red into brown-yellow, and present the widespread and yellow sediment-like turbid. Fiber strands in different size in the vitreous bodies appear in all groups. After 28 days, the proliferative fiber strands became thicker than before in control group and have more strands, while the fiber stands became thinner than before in others groups and have less fiber strands.Conclusions: 1.TMP and Ciprofloxacin inhibit the proliferation of human conjunctiva fibroblast cells in a dose dependent manner .2. A single intravitreal injection of TMP 8mg/0.4ml or Ciprofloxacin 3mg/0.1ml was nontoxic to rabbit ocular structures, as demonstrated by light microscopic examination. A intravitreal injection of ciprofloxacin 12mg/0.4ml had poison to rabbit ocular structures. 3. Intravitreal injiction of TMP and Ciprofloxacin can inhibit experimental PVR. TMP or Ciprofloxacin are potential to become adjunctive drugs as an addition to vitreoretinal surgery.4.It should be further studied in the medicin`s safe dosage scope,best administer method and so on...
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