The Study Of The Application Of PCR-Dot Blot In The Diagnosis Of The Chronic Prostatitis

Objective:In order to detect bacterial of EPS from the patients who suffering from chronic prostatitis ,we established PCR-Dot blot for 16SrRNA gene. The aim of this study is to research bacteriological etiology of the chronic prostatitis and to direct and treatment of the chronic prostatitis.Methods: Through testing the DNA of bacteria, virus and fungus with PCR and Dot blot, we established and optimized a set of efficient methods of PCR-Dot blot. At meantime, using above methods, we detected the samples of the experiment group(58 samples) and the control group(30 samples) by PCR. PCR product of the positive samples were detected by Dot blot for distinguishing gram negative bacteria and gram ositive bacteria. Results: By optimizing the PCR reaction system, the sensitive concentration detected could be 103/mL. The positive rate of the experiment group is 46.6%(27/58) with PCR, includes four positive samples with culture. In the experiment group, the positive rate is 7.0%(4/58) with culture. In the control group the ositive rate is10%(3/30) with PCR. The positive samples in two groups were detected by Dot blot. The result shows that 20 samples were gram negative and 7 samples were gram positive in the experiment group. Nevertheless, all 3 positive samples were gram positive in the control group. Conclusion: PCR-Dot blot for 16SrRNA gene could detect EPS of the chronic prostatitis for researching the bacteriological etiology and distinguish the gram negative bacteria and the gram positive bacteria. This method has many advantages, such as rapid, convenient, steady, uninfluenced, et al. It could be consider as one effective method in the diagnosis of bacterial and nonbacterialprostatitis. The results show gram negative is predominant bacteria in the bacteriological etiology of the chronic prostatitis.