Comparison Of Effects On Cytokines In Different Liquid Resuscitations For Hemorrhage Shock

PURPOSE: To investigate the changes of tumor necrosis factor-alpha(TNF-α)and interleukin-6 (IL-6) in canine acute hemorrhage model treated with 6%hydroxyethyl starch(HES) and lactated Ringer's Solution(LR) in order tounderstand the effects on inmmune system of liquid resuscitation.METHOD: 12 healthy male canines were divided into 2 groups randomly with 6canines in each group. The canine acute hemorrhage model was set up afteranesthesia. 2 groups were treated with equal amount of 6% HES and 3 folds of LRas that of the blood lost in hemorrhage respectively. Plasma TNF-αand IL-6 weredetected by ELISA at time points of femoral phlebotomy , later stage ofhemorrhagic shock ( 90 minutes after exsanguinations), resuscitation completion, 1hour and 2 hours after resuscitation.RESULT All two groups showed dramatic increases in TNF-αand IL-6 afterhemorrhagic shock ( P < 0.01 ). The plasma TNF-αand IL-6 continually elevatedduring the course of resuscitation and reach a peak in 1 hour after resuscitation.However, the increased ranges in group treated with LR was remarkably higherthan the group treated with HES and the control group ( P < 0.01 ).CONCLUSION: Cytokines such as TNF-αand IL-6 are remarkably elevated 3广西医科大学 2001 级硕士研究生学位论文during the course of shock and the consequent liquid resuscitation. Solelyintravenous infusion of lactated Ringer's solution has greater effects on theproinflammatory response after hemorrhage shock than the solely hydroxyethylstarch infusion.