| Homocysteine ( Hcy) is a kind of non - essential amino acid having sulfate group. It is the intermediary metabolite of acimetion. Recent researches show that Hcy is a king of amino acid which may reflect the injure of blood vessels. Mild to moderate hyperhomocysteinemia has been thought to be an independent risk factor of cardiovascular diseases. The macroangopathy complication of type 2 diabete is the main cause of death and mutilation. At present, it is thought that the main factor of macroangopathy complication of diabete is abnormal metabolism of protein and lipid, hemodynamics alteration, for example hyperten-tion. But there are few research about the effect of abnormal metabolism of protein and amino acid on macroangopathy of diabete. Our research tend to study the correlation of plasma homocysteine ( Hcy ) and macroangopathy in type 2 diabetes.Methods1. Study objects;Group of healthy control: 22 healthy person from out-patient clinic (12 men and 10 women) , who didnt suffering from heart, brain, liver, kidney diseases.Group of type 2 diabetes: including 23 patients (16 men and 7 women) from in - patient or outpatient clinic, who were suffering from type 2 diabetes and without vascular complication of the heart, brain, or peripheral vessels.Group of type 2 diabetes with macroangopathy: 31 patients (16 men and 15 women) who were suffering from coronary heart disease, cerebrovascular lesion,or peripheral vascular diseases. All study objects didnt take folic acid, vitamin B12 vitamin B6 6 months before the experiment.2. Test methods:Plasma Hey levels were measured by medical immunoanalysi assay.3. statistical analysisExperimental data were dealt with SPSS 10. 0 statistical analysis software in order to describe and analyze the t test, analysis of variance, x2 test, and do the correlated analysis of data.Consequence1. General condition and the indexes of diabete group:there are no significant difference in age, sex, body mass index, waist - to - hip ratio, blood pressure and renal function. The plasma Hcy level of diabete group were significantly higher than the normal control, and the plasma Hcy level of the group who were suffering from macrpangopathy compliction were significantly higher than the group who didnt have the complication. The hyperhomo-cysteinemia ( Hhcy) is definited as mean value of Hcy of the normal control plus double standard deviation. The prevalence of Hhcy of the diabete group who had macroangopathy complication was 61.90% , significantly higher than the normal control(4. 55% ) and the diabete group who didnt having macroangopathy (21. 74% ).2. The general condition and indexes of diabete groups having normal Hcy or Hhcy.To study the effect of Hhcy on macroangopathy complication of diabete, we divided the diabete groups into Hhcy group and normal Hcy group. The two groups had no significant difference in age, sex, course of disease, body mass index, prevalence of smoking, blood sugar, blood lipid. The normal Hcy group had a prevalence of macroangopathy of 21.74% , while the Hhcy group 61.9% , just the 2. 85 timese of the normal control.3 Multiple factor analysis of plasma Hcy:Taking Hcy level as dependent variable and age, course of disease, bodymass index, waist - to - hip ratio, blood pressure, blood lipid, renal function, HB ALc, blood sugar of fast and 2 hours after eating, insulin, C peptide as independent variable, we took the multiple stepwise regression. Our result showed that Hcy only positively correlated with fasting insulin and C peptide ( r = 0. 516, 0.409 and p =0.000, 0.002 respectively). The result showed that fasting insulin level was an independent factor of Hcy.4. Risk factors of macroangopathy of diabetetaking macroangopathy complication of diabete as dependent variable and course of disease, blood pressure, blood lipid, HB Alc, Hey as independent variable, we did the mutiple stepwise regression. The result showed that TG P =0. 032, Hcy P =0. 049. It showed that plasma Hcy level in an independent fact...
|
PDF Full Text Request