An Experimental Study On The Effects And Mechanisms Of Duhuojisheng Decoction In Inhibiting Carcinoma

Object: Although modern medical treatment of tumor has been developing rapidly,there are still some dissatisfactions at present. However, TCM and Integrated Traditional Western Medicine treatment of tumor have been gradually coming to ripe, and tremendous potentiality in prevention and treatment of tumor in very long history. They have showed distinctive preponderance and tremendous potentiality in prevention and treatment of tumor, and are able to compensate some misplays of modern medical treatment of tumor. Duhuojisheng decoction is an universal prescription used to treat wind-cold-damp obstruction with weakness. It has efficacies of dispelling wind and dampness.relieving pain,nourishing liver and kidney,invigorating qi and blood. Prof. Wang Sanhu thanks that the essential pathomechanism between ostealgia of cancer transfer and therapy of Duhuojisheng decoction may match. It's confirmed that Duhuojisheng decoction may lighten pathomechanism on cancer transfer through his perennial clinical practice. Therefore, the present experiment is intended to study the effects and mechanisms of Duhuojisheng decoction in inhibiting carcinoma.Methods: 1)Preparation of Duhuojisheng decoction,the optimal extracting technology was selected using the total polysaccharides and therate of paste as the standards by orthogonal design. Phenol-sulfuric acid method was used to determine the content of total polysaccharides. 2) Acute toxicity test:Gave experiment mice different concentration of Duhuojisheng decoction,then determined the maximum tolerant dose. 3)In the inhibitory experiment of the solid carcinoma: The model of mice bearing carcinoma was established by inoculating sarcoma-180 cells into the subcutaneous tissue or the abdominal cavity of the Kunming mice.The Kunming mice bearing sarcoma-180 cells were divided into five groups:high dose group of Duhuojisheng decoction;medium dose group of Duhuojisheng decoction;low dose group of Duhuojisheng decoction;5-Fu group;control group(NS group).The various drugs and natural saline were given into mice stomach at the 6th day after inoculating sarcoma-180 cells.Then ten days later,execute the mice,cut the tumor,weigh it and calculate the inhibition ratios of the tumor weight. Meanwhile,tumor tissue dissected and separated, pathological section observed in microscope. 4) Experiment on effect of Duhuojisheng decoction on immune function in inhibiting mice carcinoma:Mice bearing tumor were executed,mice thymus and spleen were cut and weighed ,spleen index and thymus index were calculated. Modified MTT assay were used to determine M activity,T-lymphocyte's generation and NK activity. Activity of IL-2 was determined by biologic activity method.Results:1 ) Preparation of Duhuojisheng decoction,the optimal conditions were as follows: The decoction was thrice, the water quantity was 10 times, 8 times and 8 times as much as the drug quantity, the boiling time was 60, 30 and 30 min in each time. 2) Acute toxicity test, maxima tolerance does of Duhuojisheng decoction for mice was 62 times of that for human body. 3) In the inhibitory experiment of the solid carcinoma, the distinction between experiment group and control grouphad a significantly meaning by analyzing average weight of the each group solid carcinoma,which indicated that the drugs of experiment group could inhibit sarcoma-180. The distinction between Duhuojisheng decoction and 5-Fu group had a significantly meaning Pathological section result indicated that tumor tissue got degeneration or necrosis in some extent and medium dose group of Duhuojisheng decoction and 5-Fu group showed more significant than NS group. 4) Experiment on effect of Duhuojisheng decoction on immune function in inhibiting mice carcinoma.(1)Effect of Duhuojisheng decoction on immunoapparatus in tumor-bearing mice. Spleen index of Duhuojisheng decoction group was enhanced. Compared with NS group, they had significant difference.Thymus index was enhanced in the medium and low dosages of Duhuojisheng decoction group compared with NS group. Spleen index an...