An Experimental Study Of Chondroregeneration In Allogeneic Transplantation Of Trachea

Objective: Trachea re-establishment following the occurrence of long and large scale trachea defects is a long unsolved problem.Trachea Substitutes can't well overcome the defects of synthetic materials. Allotransplamation of trachea can meet the standards of tracheal restoration materials.but can't satisfactorily solve the problem of secondary neerosis caused by acute ischemia following transplantation.Based on the experimental study of trachea transplantation blood circulation establishment and pathological study of experimental trachea transplantation, this paper adopted the method of fixing canine heterogeneous trachea with built-in silica gel supporting tube, and wrapped with omentum majus. in abdomenal cavity so that the observation could be performed of tracheal chondronecrosis and chondroregeneration under the condition of better establishment of tracheal blood circulation.Methods: In this experimental, 10 healthy adult rabbits were used to receive heterogeneous tracheas of healthy adult rabbits. Transplanted segment of trachea was stripped clean of adventitia ,and rinsed in penicillin saline .Equal-length silica gel tube (outer diameter 8mm)was put in transplanted trachea as a support, which was wrapped with majus and rixed in the abdomenal cavity of the receiver rabbit. Experimental rabbits were randomly divided into 5 groups. Transplanted tracheas were taken out for pathological examination 10 days?20days?30days?40days and 80days respectively after operations. Partial cartilage samples undertook transmission electron microscopy. In control group. trachea samples were embedded under skin. and taken out at corresponding times for pathological examination.Results:pedunculated omentum majus-cirded trachea transplantation ensured an early re-establishment of blood circulation of transplanted trachea and maintenance of its normal animate states; allogeneoilc transplantation of trachea activated body's immune mechanism. resulting in a chronic process of rejection and finally leading to progressive necrosis of transplanted trachea tissues: chondroregeneration occurred at every period under perichondrium after transplantation. and the degree of regeneration was related with favorable blood circulation; the emplogyment of a supporting tube in trachea,under the condition of favorable re-establishment of early blood circulation, could preventgranulation tissue hyperplasia and keep trachea cavity unobstructed.Postgraduate Ding XiaoQuan (Department of Thoracic surgery) Directed by-Lin LeShen...