Expressions Of Vascular Endothelial Growth Factor, Basic Fibroblastic Growth Factor And PCNA In Condyloma Acuminata

Obiective: To investigate the expressions and distributions of two important pro-angiogenic factors, vascular endothelial growth factor (VEGF), basic fibroblastic growth factor(bFGF) and proliferating cell nuclear antigen (PCNA) in condyloma acuminata(CA) and their significance.Methods: The expressions and distributions of VEGF, bFGF and PCNA in 35 CA specimens and 10 normal human skins were detected by SP immunohistochemistry technique.The blood vessels stained by CD34 antibody were observed in the same specimens.Results:(1) The expressions of VEGF and bFGF were both significantly higher in CA than those in normal skins.The positive expressions of VEGF and bFGF in nomal skins were both mainly located in basal layer, while in CA were both throughout epidermis with different staining patterns.The expression of VEGF was intense in basal and spinous layersjow in granular layer,while that of bFGF was low in basal layer and gradually intense from spinous to granular layer, markedly in koilocytes located in granular layer. (2) The expression of PCNA was significantly higher in CA than that in normal skins and the positive cells in CA were mainly throughout basal and spinous layers. (3) There was correlationbetween the expression of VEGF and that of PCNA, not between bFGF and PCNA in CA.(4)The blood vessels stained by CD34 antibody were much in OA than in normal skins and were dilated.Conclusions: (1) VEGF and bFGF produced by keratinocytes in nomal skin may maintain the normal physiological function of blood vessels in dermls. (2) More VEGF and bFGF produced by keratinocytes in CA as a medium may promote new blood vessel formation in dermis. (3) Hypoxia may be the important cause of overexpression of VEGF in CA.(4) The proliferation process of HPV may have some effect on the expression of bFGF in CA.